Comparison of adult somatic and excretory-secretory antigens in enzyme-linked immunosorbent assay for serodiagnosis of human infection with Fasciola gigantica

• Published in: Southeast Asian journal of tropical medicine and public health Vol. 27; no. 3; pp. 566 - 569
• Main Authors: MALEEWONG, W, INTAPAN, P. M, WONGKHAM, C, TOMANAKAN, K, DAENSEEKAEW, W, SUKEEPAISARNJAROEN, W
• Format: Journal Article
• Language: English
• Published: Bangkok Southeast Asian Ministers of Education Organization, Regional Tropical Medicine and Public Health Network 01-09-1996
• Subjects: Animals; Antigens, Helminth - analysis; Biological and medical sciences; Diseases caused by trematodes; Distomatoses; Enzyme-Linked Immunosorbent Assay - methods; Fasciola - immunology; Fascioliasis - diagnosis; Helminthic diseases; Humans; Infectious diseases; Medical sciences; Parasitic diseases; Sensitivity and Specificity; Serologic Tests - methods; Statistics, Nonparametric; Tropical medicine; Thailand; Asia; Human; Somatic antigen; Distomatosis; Trematode disease; Plathelmintha; Fasciola gigantica; Serology; Parasitosis; Helminthiasis; Trematoda; Infection; Fasciolasis; Helmintha; Diagnosis; Invertebrata; ELISA assay; Comparative study
• ISSN: 0125-1562

Adult somatic antigen extract of Fasciola gigantica was compared with excretory-secretory (ES) antigen in an enzyme-linked immunosorbent assay (ELISA) for serodiagnosis of human fascioliasis gigantica. The absorbance values in ELISA using the adult somatic antigen were not significantly different from the values obtaining using ES antigen (p > 0.05). The diagnostic sensitivity, specificity and positive and negative predictive values of the test using adult somatic extract as antigen were 100%, 98%, 70% and 100%, respectively. On the other hand, these values of the test using adult ES antigen were 100%, 99.3%, 87.5% and 100%, respectively. It appears that both somatic and ES antigens are effective antigens for use in the serodiagnosis of human fascioliasis gigantica.