A cerebrospinal fluid microRNA signature as biomarker for glioblastoma
To develop a cerebrospinal fluid (CSF) miRNA diagnostic biomarker for glioblastoma. Glioblastoma tissue and matched CSF from the same patient (obtained prior to tumor manipulation) were profiled by TaqMan OpenArray Human MicroRNA Panel. CSF miRNA profiles from glioblastoma patients and controls were...
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| Published in: | Oncotarget Vol. 8; no. 40; pp. 68769 - 68779 |
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| Main Authors: | , , , , , , , , , , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
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United States
15-09-2017
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| Abstract | To develop a cerebrospinal fluid (CSF) miRNA diagnostic biomarker for glioblastoma.
Glioblastoma tissue and matched CSF from the same patient (obtained prior to tumor manipulation) were profiled by TaqMan OpenArray
Human MicroRNA Panel. CSF miRNA profiles from glioblastoma patients and controls were created from three discovery cohorts and confirmed in two validation cohorts.
miRNA profiles from clinical CSF correlated with those found in glioblastoma tissues. Comparison of CSF miRNA profiles between glioblastoma patients and non-brain tumor patients yielded a tumor "signature" consisting of nine miRNAs. The "signature" correlated with glioblastoma tumor volume (
=0.008). When prospectively applied to cisternal CSF, the sensitivity and specificity of the 'signature' for glioblastoma detection were 67% and 80%, respectively. For lumbar CSF, the sensitivity and specificity of the signature were 28% and 95%, respectively. Comparable results were obtained from analyses of CSF extracellular vesicles (EVs) and crude CSF.
We report a CSF miRNA signature as a "liquid biopsy" diagnostic platform for glioblastoma. |
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| AbstractList | PURPOSETo develop a cerebrospinal fluid (CSF) miRNA diagnostic biomarker for glioblastoma. EXPERIMENTAL DESIGNGlioblastoma tissue and matched CSF from the same patient (obtained prior to tumor manipulation) were profiled by TaqMan OpenArray® Human MicroRNA Panel. CSF miRNA profiles from glioblastoma patients and controls were created from three discovery cohorts and confirmed in two validation cohorts. RESULTSmiRNA profiles from clinical CSF correlated with those found in glioblastoma tissues. Comparison of CSF miRNA profiles between glioblastoma patients and non-brain tumor patients yielded a tumor "signature" consisting of nine miRNAs. The "signature" correlated with glioblastoma tumor volume (p=0.008). When prospectively applied to cisternal CSF, the sensitivity and specificity of the 'signature' for glioblastoma detection were 67% and 80%, respectively. For lumbar CSF, the sensitivity and specificity of the signature were 28% and 95%, respectively. Comparable results were obtained from analyses of CSF extracellular vesicles (EVs) and crude CSF. CONCLUSIONWe report a CSF miRNA signature as a "liquid biopsy" diagnostic platform for glioblastoma. To develop a cerebrospinal fluid (CSF) miRNA diagnostic biomarker for glioblastoma. Glioblastoma tissue and matched CSF from the same patient (obtained prior to tumor manipulation) were profiled by TaqMan OpenArray Human MicroRNA Panel. CSF miRNA profiles from glioblastoma patients and controls were created from three discovery cohorts and confirmed in two validation cohorts. miRNA profiles from clinical CSF correlated with those found in glioblastoma tissues. Comparison of CSF miRNA profiles between glioblastoma patients and non-brain tumor patients yielded a tumor "signature" consisting of nine miRNAs. The "signature" correlated with glioblastoma tumor volume ( =0.008). When prospectively applied to cisternal CSF, the sensitivity and specificity of the 'signature' for glioblastoma detection were 67% and 80%, respectively. For lumbar CSF, the sensitivity and specificity of the signature were 28% and 95%, respectively. Comparable results were obtained from analyses of CSF extracellular vesicles (EVs) and crude CSF. We report a CSF miRNA signature as a "liquid biopsy" diagnostic platform for glioblastoma. |
| Author | Li, Hongying Galasko, Douglas R Mao, Ying Carter, Bob S Hochberg, Fred Akers, Johnny C Miller, Brittney Piccioni, David Chen, Clark C Yang, Zixiao Quan, Kai Zhu, Wei Ringel, Florian Li, Jie Hirshman, Brian R Messer, Karen Figueroa, Javier Ramakrishnan, Valya Komotar, Ricardo Hua, Wei |
| Author_xml | – sequence: 1 givenname: Johnny C surname: Akers fullname: Akers, Johnny C organization: Center for Theoretical and Applied Neuro-Oncology, University of California, San Diego, CA, USA – sequence: 2 givenname: Wei surname: Hua fullname: Hua, Wei organization: Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China – sequence: 3 givenname: Hongying surname: Li fullname: Li, Hongying organization: Biostatistics Department, Moores Cancer Center, UC San Diego Health System, La Jolla, CA, USA – sequence: 4 givenname: Valya surname: Ramakrishnan fullname: Ramakrishnan, Valya organization: Center for Theoretical and Applied Neuro-Oncology, University of California, San Diego, CA, USA – sequence: 5 givenname: Zixiao surname: Yang fullname: Yang, Zixiao organization: Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China – sequence: 6 givenname: Kai surname: Quan fullname: Quan, Kai organization: Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China – sequence: 7 givenname: Wei surname: Zhu fullname: Zhu, Wei organization: Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China – sequence: 8 givenname: Jie surname: Li fullname: Li, Jie organization: Center for Theoretical and Applied Neuro-Oncology, University of California, San Diego, CA, USA – sequence: 9 givenname: Javier surname: Figueroa fullname: Figueroa, Javier organization: Center for Theoretical and Applied Neuro-Oncology, University of California, San Diego, CA, USA – sequence: 10 givenname: Brian R surname: Hirshman fullname: Hirshman, Brian R organization: Center for Theoretical and Applied Neuro-Oncology, University of California, San Diego, CA, USA – sequence: 11 givenname: Brittney surname: Miller fullname: Miller, Brittney organization: Center for Theoretical and Applied Neuro-Oncology, University of California, San Diego, CA, USA – sequence: 12 givenname: David surname: Piccioni fullname: Piccioni, David organization: Department of Neurosurgery, Moores Cancer Center, UC San Diego Health System, La Jolla, CA, USA – sequence: 13 givenname: Florian surname: Ringel fullname: Ringel, Florian organization: Department of Neurosurgery, Klinikum rechts der Isar, Technische Universität München, Munich, Germany – sequence: 14 givenname: Ricardo surname: Komotar fullname: Komotar, Ricardo organization: Department of Neurological Surgery, Miller School of Medicine, University of Miami, Miami, FL, USA – sequence: 15 givenname: Karen surname: Messer fullname: Messer, Karen organization: Biostatistics Department, Moores Cancer Center, UC San Diego Health System, La Jolla, CA, USA – sequence: 16 givenname: Douglas R surname: Galasko fullname: Galasko, Douglas R organization: Department of Neurosciences, University of California, San Diego, CA, USA – sequence: 17 givenname: Fred surname: Hochberg fullname: Hochberg, Fred organization: Center for Theoretical and Applied Neuro-Oncology, University of California, San Diego, CA, USA – sequence: 18 givenname: Ying surname: Mao fullname: Mao, Ying organization: State Key Laboratory of Medical Neurobiology, Institutes of Brain Science, The Collaborative Innovation Center for Brain Science, Fudan University, Shanghai, China – sequence: 19 givenname: Bob S surname: Carter fullname: Carter, Bob S organization: Center for Theoretical and Applied Neuro-Oncology, University of California, San Diego, CA, USA – sequence: 20 givenname: Clark C surname: Chen fullname: Chen, Clark C organization: Center for Theoretical and Applied Neuro-Oncology, University of California, San Diego, CA, USA |
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| Snippet | To develop a cerebrospinal fluid (CSF) miRNA diagnostic biomarker for glioblastoma.
Glioblastoma tissue and matched CSF from the same patient (obtained prior... PURPOSETo develop a cerebrospinal fluid (CSF) miRNA diagnostic biomarker for glioblastoma. EXPERIMENTAL DESIGNGlioblastoma tissue and matched CSF from the same... |
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| Title | A cerebrospinal fluid microRNA signature as biomarker for glioblastoma |
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