GalNAc beta 1----3 terminated glycosphingolipids of human erythrocytes

• Published in: Archives of biochemistry and biophysics Vol. 251; no. 2; p. 440
• Main Authors: Angström, J, Karlsson, H, Karlsson, K A, Larson, G, Nilson, K
• Format: Journal Article
• Language: English
• Published: United States 01-12-1986
• Subjects: Acetylation; Chromatography, Gas; Chromatography, Thin Layer; Erythrocytes - metabolism; Glycosphingolipids - blood; Humans; Magnetic Resonance Spectroscopy; Mass Spectrometry; Methylation; Peptide Fragments - analysis
• ISSN: 0003-9861
• DOI: 10.1016/0003-9861(86)90350-4

Nonacid glycosphingolipids with 4 to 10 sugar residues isolated from pooled erythrocytes of blood group O donors have been efficiently separated as peracetylated derivatives on silicic acid. This procedure enabled a quantitative estimate of individual compounds and also revealed several GalNAc beta 1---3 terminated structures. The structural characterization of these glycolipids with 1H-NMR spectroscopy, direct inlet mass spectrometry, gas chromatography, and gas chromatography-mass spectrometry identified the compounds as GalNAc beta 1---3Gal alpha 1---4Gal beta 1---4Glc beta 1---1-N-acetyl sphingosine and GalNAc beta 1---3Gal alpha 1---4Gal beta 1---4Glc beta 1---1-N-acetyl phytosphingosine, GalNAc beta 1---3GalNAc beta 1---3Gal alpha 1---4Gal beta 1---4Glc beta 1---1 ceramide, and GalNAc beta 1---3Gal beta 1---4GlcNAc beta 1---3Gal beta 1---4Glc beta 1---1 ceramide.