Synthesis and immunochemical study of the antigenic determinant of the H-2Db molecule of the murine major histocompatibility complex

• Published in: Bioorganicheskaia khimiia Vol. 12; no. 1; p. 89
• Main Authors: Iurovskiĭ, V V, Safonova, N G, Khaĭdukov, S V, Vasilov, R G, Demin, V V
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01-01-1986
• Subjects: Animals; Antibodies - immunology; Antibody Specificity; Chemical Phenomena; Chemistry; Epitopes - analysis; H-2 Antigens - analysis; H-2 Antigens - immunology; Immunoenzyme Techniques; Mice; Mice, Inbred Strains; Oligopeptides - chemical synthesis; Oligopeptides - immunology; Rabbits
• ISSN: 0132-3423

Primary structure of murine class I histocompatibility antigens has been analysed to select possible antigenic determinant. Hexapeptide Leu-Gln-Gln-Leu-Ser-Gly, homologous to the region 95-100 of the H-2Db antigen heavy chain, was synthesised by stepwise elongation of peptide chain beginning from the COOH-terminal Gly. Rabbit anti-hexapeptide antibodies were obtained and shown to interact specifically with purified H-2Db antigen as well as with the native antigen on cell surface. These antibodies bind to lymphocytes of H-2b haplotype (C57BL/6 mice) but not H-2d (BALB/c) or H-2k (CBA). These data suggest that the region 95-100 is responsible for serologic differences between the alleles of H-2 antigens, i.e. it may be a xenotypic as well as an allotypic antigenic determinant. The latter was confirmed by study of interaction of the hexapeptide with allogeneic monoclonal antibodies specific to H-2Db antigen.