Growth of rat fibroblasts in tissue culture as affected by trypsin immobilized on polyurethane substrates

• Published in: Ukrainskij biohimičeskij žurnal Vol. 51; no. 4; p. 355
• Main Authors: Pkhakadze, G A, Galatenko, N A, Lipatova, T Z, Iatsenko, V P, Konoplitskaia, K L
• Format: Journal Article
• Language: Russian; Ukrainian
• Published: Ukraine 01-07-1979
• Subjects: Animals; Cell Division - drug effects; Cells, Cultured; Enzymes, Immobilized - pharmacology; Fibroblasts - physiology; Polyurethanes; Rats; Trypsin - pharmacology
• ISSN: 0201-8470

The growth of fibroblasts of the rat subcutaneous tissue was studied as affected by different doses of trypsin, free and bound, on polyurethane substrates. The method of tissue culture provides essential criteria for estimating the degree of bioincompatibility of polymeric alloimplants in particular when immobilizing biologically active substances on them. Trypsin adsorbed on the polyurethane substrate diffusing gradually into the culture medium is established to inhibit the cell growth. Chemically bounded trypsin in the composition of the polymeric matrix at the early stages of cultivation stimulates for a long time the division of fibroblastic elements and further accelerates their degeneration. It is shown that on polymeric substrates containing no trypsin, the growth character and dynamics of the fibroblastic elements are similar on the whole to these indices for cultures grown in the plasm clot without the substrate.