Conversion of phytosterols into androstenedione by Mycobacterium neoaurum

• Published in: Applied biochemistry and microbiology Vol. 44; no. 1; pp. 48 - 54
• Main Authors: Rodina, N V, Molchanova, M A, Voishvillo, N E, Andryushina, V A, Stytsenko, T S
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Nature B.V 01-01-2008
• Subjects: Bacteria; Biotechnology; Cholesterol; Fermentation; Microbiology; Mycobacterium neoaurum
• ISSN: 0003-6838; 1608-3024
• DOI: 10.1007/s10438-008-1008-0

Optimum conditions for transformation of phytosterols by Mycobacterium neoaurum, required for selective cleavage of the lateral chain into androstenedione, were shown to differ from the known conditions of animal sterol (cholesterol) transformation. Complete conversion of phytosterols into androstenedione at a substrate load of no less than 20 g/l was achieved on increasing the amount of the inoculum and the concentration of glucose (by 2 and 4 times, respectively, relative to cholesterol) and performing the fermentation under conditions of turbulent mixing. Under these conditions, both the rate of the transformation and the yield of the reaction product were high, due to the saturation of the culture liquid with hydrocarbonate. Data from the literature show that this ion is involved in cleavage of the branched lateral chain at carbon in position 24. [PUBLICATION ABSTRACT]