Endotoxin tolerance of mice to the effect of lipopolysaccharide and lipopolysaccharide-mice toxin complex of virulent strain Yersinia pestis 231

• Published in: Žurnal mikrobiologii, ėpidemiologii i immunobiologii no. 5; p. 74
• Main Authors: Ziuzina, V P, Demidova, G V, Sokolova, E P, Psiukova, N I, Bespalova, I A, Borodina, T N, Tynianova, V I
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01-09-2013
• Subjects: Animals; Immune Tolerance; Lipopolysaccharides - immunology; Lipopolysaccharides - toxicity; Mice; Yersinia pestis - immunology
• ISSN: 0372-9311

Comparative study of the effect of endotoxin tolerance of mice to the effect of lipopolysaccharide (LPS37) and complex of lipopolysaccharide with mice toxin (LPS37-MT) of a virulent Yersinia pestis 231 strain.
Preparations of LPS of highly virulent strain Y. pestis 231 obtained by phenol method from cells cultivated at 37 degrees C as well as commercial preparations of S-LPS and R-LPS of Escherichia coli were used. Mice toxin was isolated from vaccine strain Y. pestis EV76. Effect of endotoxin tolerance was determined in mice treated with aminosugar D-galactosamine.
The effect of initial LPS37 and modified form LPS37-MT of Y. pestis 231 was established to significantly differ from each other. When Y. pestis LPS37 is combined with heterologous forms--E. coli LPS or Y. pestis LPS37-MT, the inflammatory response of the organism differs and varies from complete or partial tolerance to complete lack thereof. For LPS37-MT complex only the sequence of administration to bioassay animals of LPS preparations is principal. In the case when primary activation is carried out by LPS37-MT and secondary--by Y. pestis LPS37 or S- and R- forms of E. coli LPS--the tolerance effect is absent. On the contrary, if LPS37-MT is used for recurrent activation against the background of all the other LPS forms including Y. pestis 231 LPS37 the inflammatory response is completely suppressed.
Tolerance of mice to effect of LPS and LPS-MT complex of virulent Y. pestis 231 strain was shown to be different.