Profiling Protein-DNA Interactions Cell-Type-Specifically with Targeted DamID

Profiling Protein-DNA Interactions Cell-Type-Specifically with Targeted DamID

Targeted DamID (TaDa) is a means of profiling the binding of any DNA-associated protein cell-type specifically, including transcription factors, RNA polymerase, and chromatin-modifying proteins. The technique is highly sensitive, highly reproducible, requires no mechanical disruption, cell isolation...

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Bibliographic Details
Published in:Methods in molecular biology (Clifton, N.J.) Vol. 2458; p. 195
Main Authors: Marshall, Owen J, Delandre, Caroline
Format: Journal Article
Language:English
Published: United States 2022
Subjects:
Chromatin - genetics
DNA - metabolism
DNA Methylation
Sequence Analysis, DNA - methods
Transcription Factors - metabolism
DamID
Transcription factors
Protein-DNA interactions
Gene transcription
Targeted DamID
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Description
Summary:Targeted DamID (TaDa) is a means of profiling the binding of any DNA-associated protein cell-type specifically, including transcription factors, RNA polymerase, and chromatin-modifying proteins. The technique is highly sensitive, highly reproducible, requires no mechanical disruption, cell isolation or antibody purification, and can be performed by anyone with basic molecular biology knowledge. Here, we describe the TaDa method and downstream bioinformatics data processing.
ISSN:1940-6029
DOI:10.1007/978-1-0716-2140-0_11