In vitro study on injectable alginate-strontium hydrogel for bone tissue engineering

• Published in: Zhongguo xiu fu chong jian wai ke za zhi Vol. 27; no. 12; p. 1499
• Main Authors: Tu, Yiji, Wu, Tianlong, Ye, Aifang, Xu, Junhuai, Guo, Fei, Cheng, Xigao
• Format: Journal Article
• Language: Chinese
• Published: China 01-12-2013
• Subjects: Alginates - chemistry; Alkaline Phosphatase - metabolism; Animals; Bone Marrow Cells - cytology; Bone Marrow Cells - metabolism; Cell Adhesion; Cell Differentiation - drug effects; Cell Proliferation - drug effects; Cells, Cultured; Collagen Type I - genetics; Collagen Type I - metabolism; Female; Hydrogels - chemistry; Male; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - metabolism; Osteogenesis; Rabbits; Strontium - chemistry; Tissue Engineering - methods; Tissue Scaffolds - chemistry; Transcription Factors - genetics; Transcription Factors - metabolism
• ISSN: 1002-1892

To investigate the application potential of alginate-strontium (Sr) hydrogel as an injectable scaffold material in bone tissue engineering.
The alginate-Sr/-calcium (Ca) hydrogel beads were fabricated by adding 2.0 wt% alginate sodium to 0.2 mol/L SrCl2/CaCl2 solution dropwise. Microstructure, modulus of compression, swelling rate, and degradability of alginate-Sr/-Ca hydrogels were tested. Bone marrow mesenchymal stem cells (BMSCs) were isolated from femoral bones of rabbits by flushing of marrow cavity. BMSCs at passage 5 were seeded onto the alginate-Sr hydrogel (experimental group) and alginate-Ca hydrogel (control group), and the viability and proliferation of BMSCs in 2 alginate hydrogels were assessed. The osteogenic differentiation of cells embeded in 2 alginate hydrogels was evaluated by alkaline phosphate (ALP) activity, osteoblast specific gene [Osterix (OSX), collagen type I, and Runx2] expression level and calcium deposition by fluorescent quantitative RT-PCR and alizarin red staining, Von Kossa