Conversion of 17 alpha-methyltestosterone to methandrostenolone by the bacterium Pimelobacter simplex VKPM Ac-1632 with the presence of cyclodextrins

Conditions of conversion of 17 alpha-methyltestosterone to methandrostenolone with the presence of modified beta-cyclodextrins (methylcyclodextrin, hydroxypropylcyclodextrin, and hydroxyethylcyclodextrin) in the steroid:cyclodextrin ratio 1:1 were studied. The experimental solutions of modified beta...

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Bibliographic Details
Published in:	Prikladnaja biohimija i mikrobiologija Vol. 44; no. 6; p. 642
Main Authors:	Druzhinina, A V, Andriushina, V A, Stytsenko, T S, Voĭshvillo, N E
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-11-2008
Subjects:	Anabolic Agents - metabolism Anabolic Agents - pharmacology Biotransformation - physiology Cyclodextrins - pharmacology Methandrostenolone - metabolism Methandrostenolone - pharmacology Methyltestosterone - metabolism Methyltestosterone - pharmacology Propionibacteriaceae - growth & development
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Summary:	Conditions of conversion of 17 alpha-methyltestosterone to methandrostenolone with the presence of modified beta-cyclodextrins (methylcyclodextrin, hydroxypropylcyclodextrin, and hydroxyethylcyclodextrin) in the steroid:cyclodextrin ratio 1:1 were studied. The experimental solutions of modified beta-cyclodextrins were prepared in deionized water with 5-7% methanol. Under the conditions found to be optimal, 1,2-dehydrogenation of 17 alpha-methyltestosterone was carried out with 2-4 g/l Pimelobacter simplex VKPM Ac-1632 biomass. At the substrate concentration 5-20 g/l, the reaction occurred for 1-15 h without any by-products. The maximum rate of methandrostenolone accumulation was observed with hydroxypropylcyclodextrin. The methylcyclodextrin solution can be reused for complete 17 alpha-methyltestosterone conversion at the concentration 5 g/l.
ISSN:	0555-1099