Identification by differential display of IL-24 autocrine loop activated by ras oncogenes

Ras signaling pathway is thought to control the expression of a subset of yet-to-be-defined genes that are crucial for cell growth and differentiation. Here we have identified by differential display a novel oncogenic Ras target gene encoding a new cytokine. Biochemical studies reveal that this cyto...

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Bibliographic Details
Published in:	Methods in molecular biology (Clifton, N.J.) Vol. 317; p. 207
Main Authors:	Tan, Zhongjia, Wang, Mai, Liang, Peng
Format:	Journal Article
Language:	English
Published:	United States 2006
Subjects:	Blotting, Western Cell Differentiation Cytokines - metabolism Deoxyribonuclease I - chemistry DNA, Complementary - metabolism Gene Expression Profiling - methods Gene Expression Regulation, Neoplastic Genes, ras - genetics Humans Interleukin-10 - metabolism Interleukins - biosynthesis Polymerase Chain Reaction ras Proteins - genetics Reverse Transcriptase Polymerase Chain Reaction RNA - chemistry RNA, Messenger - metabolism Time Factors
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Summary:	Ras signaling pathway is thought to control the expression of a subset of yet-to-be-defined genes that are crucial for cell growth and differentiation. Here we have identified by differential display a novel oncogenic Ras target gene encoding a new cytokine. Biochemical studies reveal that this cytokine, which we named IL-24, is a member of IL-10 family of cytokines, and it signals through two hetorodimeric receptors, whose expression is also upregulated by ras oncogenes. Thus, IL-24 and its receptors may represent a novel autocrine loop coordinately activated by ras oncogenes.
ISSN:	1064-3745