Analysis on karyotypes of ANKA strain of Plasmodium berghei

To analyze the molecular karyotypes of ANKA strain of Plasmodium berghei and demonstrate the size and number of chromosomes. To isolate the genome DNA of P. berghei ANKA strain and analyze molecular karyotypes through CHEF-III pulsed field gel electrophoresis (PFGE). The number of chromosomes was fo...

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Bibliographic Details
Published in:	Zhongguo jishengchongxue yu jishengchongping zazhi Vol. 19; no. 3; p. 176
Main Authors:	Chen, Y D, Zhang, J X, Ling, B Y
Format:	Journal Article
Language:	Chinese
Published:	China 2001
Subjects:	Animals Drug Resistance Electrophoresis, Gel, Pulsed-Field Karyotyping Mice Plasmodium berghei - classification Plasmodium berghei - genetics
Online Access:	Get more information
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Summary:	To analyze the molecular karyotypes of ANKA strain of Plasmodium berghei and demonstrate the size and number of chromosomes. To isolate the genome DNA of P. berghei ANKA strain and analyze molecular karyotypes through CHEF-III pulsed field gel electrophoresis (PFGE). The number of chromosomes was found to be 14, and their size ranged from 0.6 Mb to 3 Mb. Chromosomes number 5 to 7 and 9 to 12 appeared co-migrated in the gel. PFGE technique is useful for analyzing the molecular karyotypes and may be also useful for further study to locate the special gene on chromosomes and carry out the genetic characters and mechanism of drug resistance.
ISSN:	1000-7423