Antioxidants for bone marrow cryopreservation

Addition of antioxidants into the preservation solution improved the cryoprotection of human bone marrow cells. The viability was studied by the growth of GM-CFC in agar culture before and after storage at -196 degrees C. All used antioxidative drugs (selenomethionine, methionine, tocopherol, penici...

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Bibliographic Details
Published in:	Folia haematologica (Leipzig, Germany : 1928) Vol. 116; no. 3-4; p. 451
Main Authors:	Matthes, G, Hübner, U, Gränz, M, Schütt, M
Format:	Journal Article
Language:	English
Published:	Germany 1989
Subjects:	Antioxidants Bone Marrow Bone Marrow Cells Cell Division Cryopreservation Ferrous Compounds Granulocytes - cytology Hematopoietic Stem Cells - cytology Humans Macrophages - cytology Methionine Penicillins Selenomethionine Tissue Preservation - methods Vitamin E
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Summary:	Addition of antioxidants into the preservation solution improved the cryoprotection of human bone marrow cells. The viability was studied by the growth of GM-CFC in agar culture before and after storage at -196 degrees C. All used antioxidative drugs (selenomethionine, methionine, tocopherol, penicillin/Fe++) increased the tolerance of the stem cells to freezing and thawing and elevated the number of surviving GM-CFC up to the twofold in comparison with that of controls. More immature colony forming cells were especially protected.
ISSN:	0323-4347