DIANA-TarBase v8: a decade-long collection of experimentally supported miRNA-gene interactions

• Published in: Nucleic acids research Vol. 46; no. D1; pp. D239 - D245
• Main Authors: Karagkouni, Dimitra, Paraskevopoulou, Maria D, Chatzopoulos, Serafeim, Vlachos, Ioannis S, Tastsoglou, Spyros, Kanellos, Ilias, Papadimitriou, Dimitris, Kavakiotis, Ioannis, Maniou, Sofia, Skoufos, Giorgos, Vergoulis, Thanasis, Dalamagas, Theodore, Hatzigeorgiou, Artemis G
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 04-01-2018
• Subjects: Animals; Database Issue; Databases, Nucleic Acid; Epistasis, Genetic; High-Throughput Nucleotide Sequencing; Humans; MicroRNAs - genetics; MicroRNAs - metabolism; Oligonucleotide Array Sequence Analysis; Sequence Analysis, RNA; User-Computer Interface
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/gkx1141

Abstract
DIANA-TarBase v8 (http://www.microrna.gr/tarbase) is a reference database devoted to the indexing of experimentally supported microRNA (miRNA) targets. Its eighth version is the first database indexing >1 million entries, corresponding to ∼670 000 unique miRNA-target pairs. The interactions are supported by >33 experimental methodologies, applied to ∼600 cell types/tissues under ∼451 experimental conditions. It integrates information on cell-type specific miRNA-gene regulation, while hundreds of thousands of miRNA-binding locations are reported. TarBase is coming of age, with more than a decade of continuous support in the non-coding RNA field. A new module has been implemented that enables the browsing of interactions through different filtering combinations. It permits easy retrieval of positive and negative miRNA targets per species, methodology, cell type and tissue. An incorporated ranking system is utilized for the display of interactions based on the robustness of their supporting methodologies. Statistics, pie-charts and interactive bar-plots depicting the database content are available through a dedicated result page. An intuitive interface is introduced, providing a user-friendly application with flexible options to different queries.