Electron-cytochemical study of Ca2+ in cotyledon cells of soybean seedlings grown in microgravity

• Published in: Journal of gravitational physiology Vol. 6; no. 1; p. P123
• Main Authors: Nedukha, O, Brown, C S, Kordyum, E, Piastuch, W C
• Format: Journal Article
• Language: English
• Published: United States 01-07-1999
• Subjects: Calcium - analysis; Cotyledon - cytology; Cotyledon - drug effects; Cotyledon - ultrastructure; Ethylenes - pharmacology; Glycine max - cytology; Glycine max - drug effects; Glycine max - ultrastructure; Peroxides - pharmacology; Plant Growth Regulators - pharmacology; Plastids; Space Flight; Starch - analysis; Thylakoids; Vacuoles - physiology; Weightlessness; NASA Experiment Number 9600001; NASA Discipline Plant Biology; Non-NASA Center
• ISSN: 1077-9248

Microgravity and horizontal clinorotation are known to cause the rearrangement of the structural-functional organization of plant cells, leading to accelerated aging. Altered gravity conditions resulted in an increase in the droplets volume in cells and the destruction of chloroplast structure in Arabidopsis thaliana plants, an enhancement of cytosolic autophagaous processes, an increase in the respiration rate and a greater number of multimolecular forms of succinate- and malate dehydrogenases in cells of the Funaria hygrometrica protonema and Chlorella vulgaris, and changes in calcium balance of cells. Because ethylene is known to be involved in cell aging and microgravity appears to speed the process, and because soybean seedlings grown in space produce higher ethylene levels we asked: 1) does an acceleration of soybean cotyledon cell development and aging occur in microgravity? 2) what roles do Ca2+ ions and the enhanced ethylene level play in these events? Therefore, the goal of our investigation was to examine of the interaction of microgravity and ethylene on the localization of Ca2+ in cotyledon mesophyll of soybean seedlings.