꿀풀하고초가 직장암 예방효소 활성에 미치는 영향

• Published in: 동의생리병리학회지 Vol. 22; no. 1; pp. 126 - 130
• Main Authors: 손윤희(Yun Hee Shon), 서재범(Jae Beom Seo,), 남경수(Kyung Soo Nam)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 2008
• Subjects: 한의학; Prunella vulgaris L; ornithine decarboxylase; cytochrome P450 1A1; quinone reductase; glutathione S-transferase; glutathione
• ISSN: 1738-7698; 2288-2529; 2283-2529

Water extract from Prunella vulgaris L. (PVW) was tested for colon cancer chemopreventive activity by measuring the activities of cytochrome P450 1A1, phase Ⅱ detoxification enzyme [quinone reductase (QR) and glutathione S-transferase (GST)] and ornithine decarboxylase (ODC) and glutathione (GSH) levels in cultured human colorectal adenocarcinoma HT-29 cells. PVW significantly inhibited 7,12-dimethylbenz[a]anthracene (DMBA)-induced cytochrome P450 1A1 activity at 10 and 50 ${\mu}g/ml$. PVW induced QR activity in a dose-dependent manner over a concentration range of $1{\sim}50\;{\mu}g/ml$. GST activity was also induced with the treatment of PVW in HT-29 cells. In addition GSH levels were increased with PVW. PVW inhibited ODC activity, a key enzyme of polyamine biosynthesis, which is enhanced in tumor promotion. These results suggest that Prunella vulgaris L. has colon cancer chemopreventive activity by inhibiting cytochrome P450 1A1 and ODC activities and by increasing phase Ⅱ enzyme activity and GSH levels.