Effects of cisplatin on mitochondrial function and autophagy-related proteins in skeletal muscle of rats

• Published in: BMB reports Vol. 54; no. 11; pp. 575 - 580
• Main Authors: Seo, Dae Yun, Bae, Jun Hyun, Zhang, Didi, Song, Wook, Kwak, Hyo-Bum, Heo, Jun-Won, Jung, Su-Jeen, Yun, Hyeong Rok, Kim, Tae Nyun, Lee, Sang Ho, Kim, Amy Hyein, Jeong, Dae Hoon, Kim, Hyoung Kyu, Han, Jin
• Format: Journal Article
• Language: Korean
• Published: 생화학분자생물학회 30-11-2021
• Subjects: Autophagy; Cancer; Cisplatin; Mitochondrial function; Skeletal muscle; Mitochondrial function; Autophagy; Cisplatin; Skeletal muscle; Cancer
• ISSN: 1976-6696; 1976-670X

Cisplatin is widely known as an anti-cancer drug. However, the effects of cisplatin on mitochondrial function and autophagy-related proteins levels in the skeletal muscle are unclear. The purpose of this study was to investigate the effect of different doses of cisplatin on mitochondrial function and autophagy-related protein levels in the skeletal muscle of rats. Eight-week-old male Wistar rats (n = 24) were assigned to one of three groups; the first group was administered a saline placebo (CON, n = 10), and the second and third groups were given 0.1 mg/kg body weight (BW) (n = 6), and 0.5 mg/kg BW (n = 8) of cisplatin, respectively. The group that had been administered 0.5 mg cisplatin exhibited a reduced BW, skeletal muscle tissue weight, and mitochondrial function and upregulated levels of autophagy-related proteins, including LC3II, Beclin 1, and BNIP3. Moreover, this group had a high LC3 II/I ratio in the skeletal muscle; i.e., the administration of a high dose of cisplatin decreased the muscle mass and mitochondrial function and increased the levels of autophagy-related proteins. These results, thus, suggest that reducing mitochondrial dysfunction and autophagy pathways may be important for preventing skeletal muscle atrophy following cisplatin administration. [BMB Reports 2021; 54(11): 575-580]