Cytotoxicity of Extracts from Alternaria alternata against Cultured Tobacco BY-2Cells

• Published in: Biocontrol Science Vol. 7; no. 2; pp. 127 - 130
• Main Authors: OH, KI-BONG, TAJIMA, YASUO, SAITO, MIKAKO, CHANG, IL-MOO, MATSUOKA, HIDEAKI
• Format: Journal Article
• Language: English
• Published: Osaka-shi The Society for Antibacterial and Antifungal Agents, Japan 20-07-2002; Japan Science and Technology Agency
• Subjects: Alternaria alternata; BY-2 cells; Cytotoxicity; Metabolite
• ISSN: 1342-4815; 1884-0205
• DOI: 10.4265/bio.7.127

The cytotoxicity of extracts from Alternaria alternata against tobacco Bright Yellow-2 (BY-2) cells was investigated by a simultaneous double-staining procedureusing fluorescein diacetate and propidium iodide. The active fraction from a 10-day-old culture filtrate of the fungus was purified by ammonium sulfate precipitation, ultrafiltration, gel filtration on Sephadex G-10, and reversed-phase HPLC, in sequence. The partially purified principle appears to be heat- and acid-stable, as neither incubation at 100°C for 30 min nor overnight treatment at pH2 altered the cytotoxicity. The cytotoxicity of the principle was abolished after treatment with proteinase K or trypsin, while the dithiothreitol treatment showed reduced cytotoxicity.