Metabolism of Tegafur in Rat Liver Observed by in vivo 19F Magnetic Resonance Spectroscopy and Chromatography

• Published in: Japanese Journal of Cancer Research Vol. 83; no. 4; pp. 387 - 391
• Main Authors: Harada, Masafumi, Nishitani, Hiromu, Koga, Keiko, Miura, Iwao, Umeno, Yukihiko
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01-04-1992; Japanese Cancer Association
• Subjects: 19F‐MRS in vivo; Alanine Transaminase - blood; Animals; Antineoplastic agents; Aspartate Aminotransferases - blood; Biological and medical sciences; Carbon Tetrachloride Poisoning - metabolism; Chromatography; Chromatography, High Pressure Liquid - methods; Fluorine; Fluorouracil - metabolism; Gas Chromatography-Mass Spectrometry - methods; General aspects; Key words; Liver - metabolism; Magnetic Resonance Spectroscopy - methods; Male; Medical sciences; Pharmacology. Drug treatments; Rat liver; Rats; Rats, Inbred WKY; Tegafur; Tegafur - metabolism; Antineoplastic agent; Rat; Liver; Rodentia; NMR spectrometry; Metabolism; Chromatography; Pyrimidine; In vivo; Vertebrata; Mammalia; Animal; Antagonist
• ISSN: 0910-5050; 1349-7006; 1876-4673
• DOI: 10.1111/j.1349-7006.1992.tb00119.x

Metabolism of tegafur in the rat liver was observed by in‐vivo19F magnetic resonance spectroscopy (MRS). After MRS observation, tegafur and q‐fluorouracil (S‐FU) in the liver were determined by a shromatographic method for comparison with the results of 19F‐MRS. Rats were divided into 3 groups: 1) CCl4‐induced liver injury group, 2) uracil combined group, 3) control group. Catabolism to fluoro‐β‐alanine was suppressed in both the liver injury group and the uracil combined group. Low peaks of 5‐FU and fluoronucleotides could be found only in the uracil combined group. The result of 19F MRS observation of each group was in agreement with the result of determination of tegafur and 5‐FU by chromatography. This showed that substances which could be observed by 19F‐MRS were in proportion to all intracelluar fluoro‐containing substances. 19F‐MRS can provide direct information on the metabolism of fluoropyimidines non‐invasivey and it might be a useful aid in choosing suitable shemotherapy for patients.