Combined Use of Ribotyping, PFGE Typing and IS431 Typing in the Discrimination of Nosocomial Strains of Methicillin-Resistant Staphylococcus aureus
We have previously reported the phenotypic characterization of methicillin-resistant Staphylococcus aureus (MRSA) clinical strains isolated in Malaya University Hospital in the period 1987 to 1989 using antibiogram, coagulase typing, plasmid profiles, and phage typing. Here, we report the analysis o...
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Published in: | MICROBIOLOGY and IMMUNOLOGY Vol. 41; no. 9; pp. 687 - 695 |
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Main Authors: | , , , |
Format: | Journal Article |
Language: | English |
Published: |
Tokyo
Blackwell Publishing Ltd
01-01-1997
Center For Academic Publications Japan Center for Academic Publications Japan |
Subjects: | |
Online Access: | Get full text |
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Summary: | We have previously reported the phenotypic characterization of methicillin-resistant Staphylococcus aureus (MRSA) clinical strains isolated in Malaya University Hospital in the period 1987 to 1989 using antibiogram, coagulase typing, plasmid profiles, and phage typing. Here, we report the analysis of the same strains with three genotyping methods; ribotyping, pulsed-field gel electrophoresis (PFGE) typing, and IS431 typing (a restriction enzyme fragment length polymorphism analysis using an IS431 probe). Ribotyping could discriminate 46 clinical MRSA strains into 5 ribotypes, PFGE typing into 22 types, and IS431 typing into 15 types. Since the differences of the three genotyping patterns from strain to strain were quite independent from one another, the combined use of the three genotyping methods could discriminate 46 strains into 39 genotypes. Thus, the powerful discriminatory ability of the combination was demonstrated. |
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Bibliography: | ark:/67375/WNG-PTVX27CM-N ArticleID:MIM01912 istex:CC23B6CFC5DAD6C04BA56F8BED7B9865857B30DD ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0385-5600 1348-0421 |
DOI: | 10.1111/j.1348-0421.1997.tb01912.x |