Combined Use of Ribotyping, PFGE Typing and IS431 Typing in the Discrimination of Nosocomial Strains of Methicillin-Resistant Staphylococcus aureus

We have previously reported the phenotypic characterization of methicillin-resistant Staphylococcus aureus (MRSA) clinical strains isolated in Malaya University Hospital in the period 1987 to 1989 using antibiogram, coagulase typing, plasmid profiles, and phage typing. Here, we report the analysis o...

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Published in:MICROBIOLOGY and IMMUNOLOGY Vol. 41; no. 9; pp. 687 - 695
Main Authors: Yoshida, Tatsumi, Kondo, Noriko, Hanifah, Yasmin Abu, Hiramatsu, Keiichi
Format: Journal Article
Language:English
Published: Tokyo Blackwell Publishing Ltd 01-01-1997
Center For Academic Publications Japan
Center for Academic Publications Japan
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Summary:We have previously reported the phenotypic characterization of methicillin-resistant Staphylococcus aureus (MRSA) clinical strains isolated in Malaya University Hospital in the period 1987 to 1989 using antibiogram, coagulase typing, plasmid profiles, and phage typing. Here, we report the analysis of the same strains with three genotyping methods; ribotyping, pulsed-field gel electrophoresis (PFGE) typing, and IS431 typing (a restriction enzyme fragment length polymorphism analysis using an IS431 probe). Ribotyping could discriminate 46 clinical MRSA strains into 5 ribotypes, PFGE typing into 22 types, and IS431 typing into 15 types. Since the differences of the three genotyping patterns from strain to strain were quite independent from one another, the combined use of the three genotyping methods could discriminate 46 strains into 39 genotypes. Thus, the powerful discriminatory ability of the combination was demonstrated.
Bibliography:ark:/67375/WNG-PTVX27CM-N
ArticleID:MIM01912
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ObjectType-Article-2
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ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1997.tb01912.x