Large variation in t(11;14)(q13;q32) and t(14;18)(q32;q21) translocation product size is confirmed by sequence analysis of PCR products
Summary Polymerase chain reaction is commonly used to detect t(11;14)(q13;q32) and t(14;18)(q32;q21) chromosomal translocations associated with mantle cell lymphoma and follicular lymphoma. We tested a total of 482 samples from patients with suspected non‐Hodgkin's lymphoma and sequenced unusua...
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| Published in: | Clinical and laboratory haematology Vol. 28; no. 4; pp. 248 - 253 |
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| Main Authors: | , , , , |
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Oxford, UK
Blackwell Publishing Ltd
01-08-2006
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| Abstract | Summary
Polymerase chain reaction is commonly used to detect t(11;14)(q13;q32) and t(14;18)(q32;q21) chromosomal translocations associated with mantle cell lymphoma and follicular lymphoma. We tested a total of 482 samples from patients with suspected non‐Hodgkin's lymphoma and sequenced unusual‐sized t(11;14)(q13;q32) and t(14;18)(q32;q21) products from 33 of these patients. BCL‐1 or BCL‐2 gene rearrangements were confirmed in 23 of 33 patients (70%). Considerable size variation was observed using t(11;14) primers, with MTCA and MTCB t(11;14) products ranging from 234 to 934 bp and 143 to 560 bp respectively. Less variability was observed for t(14;18) Major Breakpoint Region (MBR) products (100–252 bp) but Minor Cluster Region (MCR) products ranged from 217 to 498 bp. We demonstrate the utility of sequence analysis to confirm unusual‐sized translocation products and reduce false‐positive results because of nonspecific amplification. |
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| AbstractList | Polymerase chain reaction is commonly used to detect t(11;14)(q13;q32) and t(14;18)(q32;q21) chromosomal translocations associated with mantle cell lymphoma and follicular lymphoma. We tested a total of 482 samples from patients with suspected non-Hodgkin's lymphoma and sequenced unusual-sized t(11;14)(q13;q32) and t(14;18)(q32;q21) products from 33 of these patients. BCL-1 or BCL-2 gene rearrangements were confirmed in 23 of 33 patients (70%). Considerable size variation was observed using t(11;14) primers, with MTCA and MTCB t(11;14) products ranging from 234 to 934 bp and 143 to 560 bp respectively. Less variability was observed for t(14;18) Major Breakpoint Region (MBR) products (100-252 bp) but Minor Cluster Region (MCR) products ranged from 217 to 498 bp. We demonstrate the utility of sequence analysis to confirm unusual-sized translocation products and reduce false-positive results because of nonspecific amplification. Summary Polymerase chain reaction is commonly used to detect t(11;14)(q13;q32) and t(14;18)(q32;q21) chromosomal translocations associated with mantle cell lymphoma and follicular lymphoma. We tested a total of 482 samples from patients with suspected non‐Hodgkin's lymphoma and sequenced unusual‐sized t(11;14)(q13;q32) and t(14;18)(q32;q21) products from 33 of these patients. BCL‐1 or BCL‐2 gene rearrangements were confirmed in 23 of 33 patients (70%). Considerable size variation was observed using t(11;14) primers, with MTCA and MTCB t(11;14) products ranging from 234 to 934 bp and 143 to 560 bp respectively. Less variability was observed for t(14;18) Major Breakpoint Region (MBR) products (100–252 bp) but Minor Cluster Region (MCR) products ranged from 217 to 498 bp. We demonstrate the utility of sequence analysis to confirm unusual‐sized translocation products and reduce false‐positive results because of nonspecific amplification. |
| Author | HARRIES, L. W. WICKHAM, C. L. SARSFIELD, P. JOYNER, M. V. ELLARD, S. |
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| References_xml | – volume: 87 start-page: 223 year: 1995 end-page: 224 article-title: Occurrence of bcl‐2 oncogene translocation with increased frequency in the peripheral blood of heavy smokers publication-title: Journal of the National Cancer Institute – volume: 7 start-page: 1437 year: 1993 end-page: 1440 article-title: Chromosome t(11;14)(q13, q32) breakpoints in centrocytic lymphoma are highly localized at the bcl‐1 major translocation cluster publication-title: Leukaemia – volume: 316 start-page: 279 year: 1987 article-title: Multiple recurrent genomic defects in follicular lymphoma publication-title: New England Journal of Medicine – volume: 9 start-page: 2133 year: 2003 end-page: 2139 article-title: The t(14;18) is associated with germinal center‐derived diffuse large B‐cell lymphoma and is a strong predictor of outcome publication-title: Clinical Cancer Research – volume: 81 start-page: 3063 year: 1993 end-page: 3067 article-title: Rearrangement and overexpression of the gene in intermediate lymphocytic lymphomas and in t(11q13)‐bearing leukemias publication-title: Blood – volume: 78 start-page: 3275 year: 1991 end-page: 3280 article-title: All advanced stage non‐Hodgkin's lymphomas with a polymerase chain reaction amplifiable breakpoint of bcl‐2 have residual cells containing the bcl‐2 rearrangement at evaluation and after treatment publication-title: Blood – volume: 78 start-page: 493 year: 1991 end-page: 498 article-title: Rearrangement of the chromosome 11 bcl‐1 locus in centrocytic lymphoma: analysis with multiple breakpoint probes publication-title: Blood – volume: 372 start-page: 143 year: 1994 end-page: 149 article-title: Chromosomal translocations in human cancer publication-title: Nature – volume: 75 start-page: 163 year: 1990 end-page: 167 article-title: Development of a highly sensitive assay, based on the polymerase chain reaction, for rare B‐lymphocyte clones in a polyclonal population publication-title: British Journal of Haematology – volume: 17 start-page: 2257 year: 2003 end-page: 2317 article-title: Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T‐cell receptor gene recombinations in suspect lymphoproliferations publication-title: Leukemia – volume: 58 start-page: 451 year: 1996 end-page: 456 article-title: Rapid detection of regionally clustered germ‐line mutations by multiplex hetroduplex analysis publication-title: American Journal of Human Genetics – volume: 53 start-page: 19 year: 2000 end-page: 23 article-title: Amplification of PCR products in excess of 600 base pairs using DNA extracted from decalcified, paraffin wax embedded bone marrow trephine biopsies publication-title: Journal of Clinical Pathology: Molecular Pathology |
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| Snippet | Summary
Polymerase chain reaction is commonly used to detect t(11;14)(q13;q32) and t(14;18)(q32;q21) chromosomal translocations associated with mantle cell... Polymerase chain reaction is commonly used to detect t(11;14)(q13;q32) and t(14;18)(q32;q21) chromosomal translocations associated with mantle cell lymphoma... |
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| SubjectTerms | 14)(q13 18)(q32 chromosomal translocation Chromosomes, Human, Pair 11 - genetics Chromosomes, Human, Pair 14 - genetics Chromosomes, Human, Pair 18 - genetics Genes, bcl-1 - genetics Genes, bcl-2 - genetics Humans Lymphoma, Non-Hodgkin - genetics Molecular Sequence Data Polymerase Chain Reaction q21 q32 Retrospective Studies sequencing t(11;14)(q13;q32) t(14;18)(q32;q21) Translocation, Genetic - genetics Translocation, Genetic - physiology |
| Title | Large variation in t(11;14)(q13;q32) and t(14;18)(q32;q21) translocation product size is confirmed by sequence analysis of PCR products |
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