Hepatotoxicity from anabolic androgenic steroids marketed as dietary supplements: contribution from ATP8B1/ABCB11 mutations?

• Published in: Liver international Vol. 33; no. 8; pp. 1266 - 1270
• Main Authors: El Sherrif, Yasser, Potts, Jonathan R., Howard, Mark R., Barnardo, Adrian, Cairns, Stuart, Knisely, Alex S., Verma, Sumita
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01-09-2013
• Subjects: Adenosine Triphosphatases - genetics; Adult; Anabolic Agents - adverse effects; Androgens - adverse effects; ATP Binding Cassette Subfamily B Member 11; ATP-Binding Cassette Transporters - genetics; benign recurrent intrahepatic cholestasis; Biomarkers - blood; Chemical and Drug Induced Liver Injury - blood; Chemical and Drug Induced Liver Injury - genetics; cholestasis; Cholestasis, Intrahepatic - blood; Cholestasis, Intrahepatic - chemically induced; Cholestasis, Intrahepatic - genetics; Dietary Supplements - adverse effects; DNA Mutational Analysis; drug-induced liver injury; gamma-Glutamyltransferase - blood; Genetic Predisposition to Disease; Humans; Hyperbilirubinemia - chemically induced; Hyperbilirubinemia - genetics; Male; Middle Aged; Mutation; Phenotype; Risk Factors; drug-induced liver injury; cholestasis; benign recurrent intrahepatic cholestasis
• ISSN: 1478-3223; 1478-3231
• DOI: 10.1111/liv.12216

Background
Though possession of androgenic anabolic steroids (AAS) is illegal, non‐prescription use of AAS persists.
Methods
We describe two Caucasian males (aged 25 and 45 years) with cholestatic hepatitis following ingestion of the dietary supplement Mass‐Drol (‘Celtic Dragon’) containing the AAS 2α‐17α‐dimethyl‐etiocholan‐3‐one,17β‐ol.
Results
Despite substantial hyperbilirubinaemia peak gamma‐glutamyl transferase (GGT) remained normal. Besides ‘bland’ intralobular cholestasis, liver biopsy in both found deficiency of canalicular expression of ectoenzymes as seen in ATP8B1 disease. In the older patient, bile salt export pump marking (encoded by ABCB11) was focally diminished. We hypothesized that AAS had either induced inhibition of normal ATP8B1/ABCB11 expression or triggered initial episodes of benign recurrent intrahepatic cholestasis (BRIC) type 1/or 2. On sequencing, ATP8B1 was normal in both patients although the younger was heterozygous for the c.2093G>A mutation in ABCB11, a polymorphism previously encountered in drug‐induced liver injury.
Conclusion
AAS marketed as dietary supplements continue to cause hepatotoxicity in the UK; underlying mechanisms may include unmasking of genetic cholestatic syndromes.