Does Background Intensity Estimation Influence the Iterative Restoration of Microscope Images?

• Published in: 2010 23rd SIBGRAPI Conference on Graphics, Patterns and Images pp. 56 - 62
• Main Authors: Ponti, M P, Mascarenhas, N D A
• Format: Conference Proceeding
• Language: English
• Published: IEEE 01-08-2010
• Subjects: background estimation; Biomedical imaging; Estimation; Histograms; Image restoration; Iterative methods; Microscopy; Noise
• ISBN: 9781424484201; 1424484200
• ISSN: 1530-1834; 2377-5416
• DOI: 10.1109/SIBGRAPI.2010.16

A new background estimation method is proposed to improve iterative restoration of microscope images. The method is based on a sequence of subtractions performed in the beginning of an iterative restoration procedure. A series of experiments using background intensity detection methods were carried out in order to analyse the influence of a correct estimation of the background. The restoration results as a function of the detected background value is discussed. The results indicates a strong influence of the estimate on the restoration of images. When compared to an optimal error-based method, the proposed method was faster and obtained a good approximation to the correct value.