Carbon isotope compositions (δ(13) C) of leaf, wood and holocellulose differ among genotypes of poplar and between previous land uses in a short-rotation biomass plantation

• Published in: Plant, cell and environment Vol. 38; no. 1; pp. 144 - 156
• Main Authors: Verlinden, M S, Fichot, R, Broeckx, L S, Vanholme, B, Boerjan, W, Ceulemans, R
• Format: Journal Article
• Language: English
• Published: United States Wiley 01-01-2015
• Subjects: Biomass; Carbon - metabolism; Carbon Isotopes - analysis; Cellulose - metabolism; Genetic Variation; Genotype; Life Sciences; Nitrogen - metabolism; Phenotype; Photosynthesis; Plant Leaves - genetics; Plant Leaves - growth & development; Populus - genetics; Populus - growth & development; Populus - physiology; Seasons; Soil - chemistry; Trees; Water - metabolism; Wood - genetics; Wood - growth & development; water use efficiency; water relations; genotypic variations; bioenergy; carbon isotope discrimination; cropland; growth; pasture; Populus spp
• ISSN: 0140-7791; 1365-3040
• DOI: 10.1111/pce.12383

The efficiency of water use to produce biomass is a key trait in designing sustainable bioenergy-devoted systems. We characterized variations in the carbon isotope composition (δ(13) C) of leaves, current year wood and holocellulose (as proxies for water use efficiency, WUE) among six poplar genotypes in a short-rotation plantation. Values of δ(13) Cwood and δ(13) Cholocellulose were tightly and positively correlated, but the offset varied significantly among genotypes (0.79-1.01‰). Leaf phenology was strongly correlated with δ(13) C, and genotypes with a longer growing season showed a higher WUE. In contrast, traits related to growth and carbon uptake were poorly linked to δ(13) C. Trees growing on former pasture with higher N-availability displayed higher δ(13) C as compared with trees growing on former cropland. The positive relationships between δ(13) Cleaf and leaf N suggested that spatial variations in WUE over the plantation were mainly driven by an N-related effect on photosynthetic capacities. The very coherent genotype ranking obtained with δ(13) C in the different tree compartments has some practical outreach. Because WUE remains largely uncoupled from growth in poplar plantations, there is potential to identify genotypes with satisfactory growth and higher WUE.