Genetic changes in intraductal breast cancer detected by comparative genomic hybridization

• Published in: The American journal of pathology Vol. 150; no. 4; pp. 1465 - 1471
• Main Authors: Kuukasjarvi, T, Tanner, M, Pennanen, S, Karhu, R, Kallioniemi, OP, Isola, J
• Format: Journal Article
• Language: English
• Published: Bethesda, MD ASIP 01-04-1997; American Society for Investigative Pathology
• Subjects: Biological and medical sciences; Breast Neoplasms - genetics; Breast Neoplasms - pathology; Carcinoma in Situ - genetics; Carcinoma in Situ - pathology; Carcinoma, Ductal, Breast - genetics; Carcinoma, Ductal, Breast - pathology; Carcinoma, Intraductal, Noninfiltrating - genetics; Carcinoma, Intraductal, Noninfiltrating - pathology; Chromosome Mapping; Female; Gynecology. Andrology. Obstetrics; Humans; In Situ Hybridization, Fluorescence; Mammary gland diseases; Medical sciences; Nucleic Acid Hybridization; Polymerase Chain Reaction; Tumors; Chromosomal aberration; Human; Immunohistochemistry; Ductal carcinoma; Carcinoma in situ; Microdissection; In situ; Fluorescence; Malignant tumor; Hybridization; Carcinogenesis; Polymerase chain reaction; Pathology; Mammary gland diseases; Mammary gland; Molecular biology; Genome
• ISSN: 0002-9440; 1525-2191

Ductal carcinoma in situ (DCIS) is considered a direct precursor of invasive ductal breast cancer (IDC). We combined tissue microdissection and comparative genomic hybridization to identify genetic changes in five DCIS lesions with no invasion and in two that were adjacent to IDC. Extensive genetic changes characterized pure DCIS cases with gains of 1q, 6q, 8q, and Xq as well as losses of 17p and chromosome 22 being most often involved. Except for the Xq gain, these changes are also common to IDC. Separate analysis of DCIS and IDC components in the same tumor revealed an almost identical pattern of genetic changes in one case, whereas substantial differences were found in another. We conclude that many of the common genetic changes in IDC may take place before development of invasive growth. However, a simple linear progression model may not always account for the DCIS-IDC transition.