Rate of uptake of CO by hemoglobin in pig erythrocytes as a function of PO2

• Published in: Journal of applied physiology (1985) Vol. 81; no. 4; pp. 1544 - 1549
• Main Authors: Te Nijenhuis, Francis C. A. M, Lin, Lydia, Moens, Gerko H, Versprille, Adrian, Forster, Robert E
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Physiological Soc 01-10-1996; American Physiological Society
• Subjects: Animals; Biological and medical sciences; Blood Volume - physiology; Carbon Monoxide - metabolism; Erythrocytes - metabolism; Female; Fundamental and applied biological sciences. Psychology; Hemoglobins - metabolism; In Vitro Techniques; Kinetics; Oxygen - blood; Regression Analysis; Species Specificity; Swine; Vertebrates: blood, hematopoietic organs, reticuloendothelial system; Blood gas; Oxygen; Red blood cell; Uptake; Pig; Blood cell; Vertebrata; Mammalia; Partial pressure; Blood volume; Hemoglobin; Blood capillary; Artiodactyla; Carbon monoxide; Ungulata
• ISSN: 8750-7587; 1522-1601
• DOI: 10.1152/jappl.1996.81.4.1544

Francis C. A. M.
Te Nijenhuis,
Lydia
Lin,
Gerko H.
Moens,
Adrian
Versprille, and
Robert E.
Forster
Department of Physiology, School of Medicine, University of
Pennsylvania, Philadelphia, Pennsylvania 19104-6085; and
Pathophysiology Laboratory, Department of Pulmonary Diseases,
Erasmus University, 3000 DR Rotterdam, The Netherlands
Received 18 April 1994; accepted in final form 22 May 1996.
Te Nijenhuis, Francis C. A. M., Lydia Lin, Gerko H. Moens,
Adrian Versprille, and Robert E. Forster. Rate of uptake of CO by
hemoglobin in pig erythrocytes as a function of
P O 2 . J. Appl.
Physiol. 81(4): 1544-1549, 1996. This study was
initiated to obtain data on the rate of carbon monoxide (CO) uptake
( CO ) by hemoglobin in pig
erythrocytes to derive, in a later study, the pulmonary capillary blood
volume (Qc) in pigs from the Roughton-Forster relationship. Blood from
five different female pigs was used. The
CO , the milliliters of CO taken
up by 1 ml of whole blood per minute per Torr CO tension, was
determined on each blood sample with a continuous-flow rapid-mixing
apparatus and double-beam spectrophotometry at 37°C and pH 7.4 at
four or five different P O 2 values.
Because the individual regression lines of CO vs.
P O 2 were not significantly different,
a common regression equation was calculated:
1/ CO  = 0.0084 P O 2 + 0.63. The slope of this
regression line is significantly steeper than the reported slopes of
the regression lines for human and dog erythrocytes measured under the
same conditions. Our results revealed that calculation of
Qc in pigs by using CO values
for human or dog erythrocytes would result in an underestimation of 51 and 50%, respectively.
carbon monoxide; partial pressure of oxygen; capillary blood
volume; continuous flow; species
0161-7567/96 $5.00
Copyright © 1996 the American Physiological Society