An alternatively spliced cadherin-11 enhances human breast cancer cell invasion

• Published in: Cancer research (Chicago, Ill.) Vol. 62; no. 22; pp. 6688 - 6697
• Main Authors: FELTES, Carolyn M, KUDO, Akira, BLASCHUK, Orest, BYERS, Stephen W
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA American Association for Cancer Research 15-11-2002
• Subjects: Adherens Junctions - metabolism; Adherens Junctions - physiology; Alternative Splicing; Antibodies, Monoclonal - biosynthesis; Antibodies, Monoclonal - immunology; Antibody Specificity; Biological and medical sciences; Breast Neoplasms - genetics; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Cadherins - biosynthesis; Cadherins - metabolism; Cadherins - pharmacology; Cadherins - physiology; Cell Adhesion - drug effects; Cell Adhesion - physiology; Cell Division - drug effects; Cell Division - physiology; Dissemination; Humans; Medical sciences; Neoplasm Invasiveness; Transfection; Tumor cell; Tumors; Human; Alternative splicing; Metastasis; Malignant tumor; Cadherin; Gene expression; In vitro; Invasion; Mammary gland diseases; Dissemination; Established cell line; Mammary gland; Mechanism of action; Tumor cell
• ISSN: 0008-5472; 1538-7445

Although reduced levels of the epithelial cell adhesion molecule E-cadherin are often associated with poorly differentiated breast cancers, recent studies show that expression of other cadherins such as N-cadherin, P-cadherin, and the mesenchymal cadherin-11 is actually elevated in invasive breast cancers and cell lines. Cadherin-11 is unique among cadherins in that it exists as two alternatively spliced forms that are expressed together in the same cell. We now show that expression of wild-type cadherin-11, with or without coexpression of the COOH-terminal truncated splice variant, promotes epithelial differentiation of the cadherin-negative SKBR3 cell line. Exogenous wild-type cadherin-11 association with and membrane recruitment of beta-catenin and p120 are unaffected by coexpression of the truncated variant. Cadherin-11-expressing cells exhibit modest changes in cell proliferation and no change in anchorage-independent growth. However, coexpression of wild-type cadherin-11 and the splice variant promotes a dramatic increase in the ability of SKBR3 cells and E-cadherin-positive MCF7 cells to traverse Matrigel-coated filters. Biochemical studies indicate that the truncated variant may be secreted from the cell and/or enter a detergent-insoluble compartment. These data suggest that the presence of the cadherin-11 splice variant promotes invasion of cadherin-11-positive breast cancer cells.