Predicting Treatment Outcome in Cervical Diseases Using Liquid-based Cytology, Dynamic HPV Genotyping and DNA Cytometry

• Published in: Anticancer research Vol. 26; no. 2B; pp. 1439 - 1446
• Main Authors: BOLLMANN, Magdolna, VARNAI, Alinda Dalma, GRIEFINGHOLT, Harald, BANKFALVI, Agnes, CALLENBERG, Helene, SPEICH, Norbert, SCHMITT, Christoph, BOLLMANN, Reinhard
• Format: Journal Article
• Language: English
• Published: Attiki International Institute of Anticancer Research 01-03-2006
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Biological and medical sciences; Cervical Intraepithelial Neoplasia - pathology; Cervical Intraepithelial Neoplasia - surgery; Cervical Intraepithelial Neoplasia - virology; Condylomata Acuminata - pathology; Condylomata Acuminata - surgery; Condylomata Acuminata - virology; Female; Flow Cytometry - methods; Genotype; Human papillomavirus; Humans; Medical sciences; Middle Aged; Neoplasm, Residual - pathology; Neoplasm, Residual - virology; Papillomaviridae - genetics; Papillomavirus Infections - pathology; Papillomavirus Infections - surgery; Papillomavirus Infections - virology; Ploidies; Polymerase Chain Reaction; Predictive Value of Tests; Treatment Outcome; Tumors; Uterine Cervical Diseases - pathology; Uterine Cervical Diseases - surgery; Uterine Cervical Diseases - virology; Uterine Cervical Neoplasms - pathology; Uterine Cervical Neoplasms - surgery; Uterine Cervical Neoplasms - virology; Vaginal Smears; Ploidy; Prognosis; DNA-ploidy; Genotype; CIN; ThinPrep cytology; Papovaviridae; Liquid based cytology; HPV genotyping; Papillomavirus; Virus; Cytometry; Human papillomavirus; Cancerology; Treatment; Dynamics; DNA
• ISSN: 0250-7005; 1791-7530

Background: In this study, our prospective experience with a multimodal follow-up protocol is summarized, with special emphasis
on predicting the treatment outcome of cervical diseases. Materials and Methods: Liquid-based cytology samples (ThinPrep)
from 209 women exhibiting the whole spectrum of human papilloma virus (HPV)-related cervical diseases were investigated by
cytology, PCR-based HPV genotyping and DNA cytometry pre-surgery. The first control cytology and type-specific HPV tests were
performed at 3 months post-surgery. Results: The success rate of surgery was 95% in eradicating high-grade cervical disease
and 90% in eliminating the baseline HPV genotype. Treatment failure was significantly correlated with baseline cytology (p=0.011),
resection margin status (p=0.016) and HPV positivity at 3 months post-surgery (p=0.04). Multivariate logistic regression analysis
showed that type-specific persistent HPV infection (p=0.028), baseline cytology (p=0.039) and histology (p=0.065) were independent
predictors of residual cervical neoplasias. Conclusion: Our results showed that our multimodal surveillance protocol may help
to individually assess the anticipated clinical outcome of cervical diseases post-surgery.