Measurement of DNA copy number at microsatellite loci using quantitative PCR analysis

This report describes the development and validation of quantitative microsatellite analysis (QuMA) for rapid measurement of relative DNA sequence copy number. In QuMA, the copy number of a test locus relative to a pooled reference is assessed using quantitative, real-time PCR amplification of loci...

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Bibliographic Details
Published in:	Cancer research (Chicago, Ill.) Vol. 60; no. 19; pp. 5405 - 5409
Main Authors:	GINZINGER, David G, GODFREY, Tony E, NIGRO, Janice, MOORE, Dan H, SUZUKI, Seiji, PALLAVICINI, Maria G, GRAY, Joe W, JENSEN, Ronald H
Format:	Journal Article
Language:	English
Published:	Philadelphia, PA American Association for Cancer Research 01-10-2000
Subjects:	Animals Biological and medical sciences Biotechnology chromosome 2 copy number DNA, Neoplasm - analysis DNA, Neoplasm - genetics Female Fundamental and applied biological sciences. Psychology Gene Dosage Genes, Tumor Suppressor - genetics Genetic engineering Genetic technics Humans Leukemia, Myeloid - etiology Leukemia, Myeloid - genetics Leukemia, Radiation-Induced - genetics Male Methods. Procedures. Technologies Mice Mice, Inbred Strains Mice, Knockout Microsatellite Repeats - genetics Modification of gene expression level Neoplasm Proteins - genetics Nucleic Acid Amplification Techniques Ovarian Neoplasms - genetics Polymerase Chain Reaction - methods Prognosis Reproducibility of Results Survival Analysis Trans-Activators - genetics ZNF217 gene Human Methodology Nucleotide sequence Copy number Rodentia Biological marker Chromosome 2 In vitro Polymerase chain reaction Tissue Vertebrata Gene amplification Mammalia Gene Comparative genomic hybridization Investigation method Mouse Animal Microsatellite DNA DNA Established cell line Onc gene Quantitative analysis
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