New Methodological Approach to Induce a Differentiation Phenotype in Caco-2 Cells Prior to Post-confluence Stage

Background: Various differentiation-inducing agents or harvesting of spontaneously late post-confluence cultures have been used to differentiate the human colon carcinoma Caco-2 cell line. We report a new procedure to generate pre-confluent subcultures of Caco-2 population at various stages of diffe...

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Published in:	Anticancer research Vol. 27; no. 6B; pp. 3919 - 3925
Main Authors:	FERRARETTO, Anita, GRAVAGHI, Claudia, DONETTI, Elena, COSENTINO, Stefania, DONIDA, Bianca M, BEDONI, Marzia, LOMBARDI, Giovanni, FIORILLI, Amelia, TETTAMANTI, Guido
Format:	Journal Article
Language:	English
Published:	Attiki International Institute of Anticancer Research 01-11-2007
Subjects:	Biological and medical sciences Caco-2 Cells - enzymology Caco-2 Cells - pathology Caco-2 Cells - ultrastructure Cell Count Cell Culture Techniques - methods Cell Differentiation - physiology Cell Growth Processes - physiology Humans Medical sciences Phenotype Tumors Cell proliferation Phenotype Clinical stage Cancerology Caco-2 cells differentiation intestine proliferation Digestive system Confluence Gut Cell differentiation
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Summary:	Background: Various differentiation-inducing agents or harvesting of spontaneously late post-confluence cultures have been used to differentiate the human colon carcinoma Caco-2 cell line. We report a new procedure to generate pre-confluent subcultures of Caco-2 population at various stages of differentiation without altering culture conditions. Materials and Methods: Ultrastructural analysis, cell proliferation activity and biochemical markers of differentiation were evaluated at different passages. Results: Subcultures of Caco-2 cells at pre-confluence, exhibiting progressive acquisition of a more benign differentiation phenotype, were generated. Early passages of Caco-2 cells showed a well-developed brush border and incomplete junctional apparatus; subsequent subcultures yielded cell populations with well-developed junctions similar to those of small intestinal cells. Conclusion: These culture conditions represent a new versatile model not only to progressively induce the differentiation program in Caco-2 cells at pre-confluence without changes of culture media, but also to explore mechanistic modes of drug transport and tumor development.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0250-7005 1791-7530