The Expression of p21/WAF-1 and Cyclin B1 Mediate Mitotic Delay in x-Irradiated Fibroblasts

Background: To better understand the relationship between mitotic delay and the disruption of cyclin B1 and p21 in x-irradiated fibroblasts, studies were carried out to establish correlations between the downregulation of cyclin B1 by the cyclin kinase inhibitor (CKI) p21 and the induction of mitoti...

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Published in:	Anticancer research Vol. 25; no. 2A; pp. 1123 - 1129
Main Authors:	CARIVEAU, Mickael J, KOVACS, Charles J, ALLISON, Ron R, JOHNKE, Roberta M, KALMUS, Gerhard W, EVANS, Mark
Format:	Journal Article
Language:	English
Published:	Attiki International Institute of Anticancer Research 01-03-2005
Subjects:	Animals Biological and medical sciences Cell Cycle - physiology Cell Cycle - radiation effects Cell Cycle Proteins - biosynthesis Cell Cycle Proteins - radiation effects Cyclin B - biosynthesis Cyclin B - radiation effects Cyclin B1 Cyclin-Dependent Kinase Inhibitor p21 Fibroblasts - cytology Fibroblasts - metabolism Fibroblasts - radiation effects Medical sciences Mice Mitosis - physiology Mitosis - radiation effects NIH 3T3 Cells Tumors X-Rays Mitosis CDKN1A Gene Irradiated mitotic delay signaling proteins Cyclin B1 Protein DSB Cancerology Signaling pathway Cell cycle Fibroblast cell cycle analysis
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Summary:	Background: To better understand the relationship between mitotic delay and the disruption of cyclin B1 and p21 in x-irradiated fibroblasts, studies were carried out to establish correlations between the downregulation of cyclin B1 by the cyclin kinase inhibitor (CKI) p21 and the induction of mitotic delay in the NIH3T3 fibroblast. Materials and Methods: Cell cycle kinetics were used to analyze mitotic delay in irradiated NIH3T3 cells and immunocytochemistry incorporated to assess the expression of cyclin B1 and p21, following 2 or 4Gy x-irradiation. Results and Discussion: Results indicate a dose dependent increase in mitotic delay accompanied by a downregulation of cyclin B1 and corresponding upregulation of the CKI p21 in exponentially growing cultures. Data indicates that the induction of radiation - induced division delay appears to be dependent on the p21 inhibition of cyclin B1 and, furthermore, p21 and cyclin B1 expression are highly dependent on cell density.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0250-7005 1791-7530