A Novel Chromatographic Method for Ep-CAM mRNA Detection in Peripheral Blood and Bone Marrow of Patients with Metastatic Colorectal Cancer

• Published in: Anticancer research Vol. 25; no. 1A; pp. 319 - 323
• Main Authors: KARANIKIOTIS, Charisios, SKIADAS, Ioannis, KARINA, Maria, GEORGAKOPOULOU, Stavroula, GEORGAKOPOULOS, Eleftherios, FOUNTZILAS, George
• Format: Journal Article
• Language: English
• Published: Attiki International Institute of Anticancer Research 01-01-2005
• Subjects: Antigens, Neoplasm - genetics; Biological and medical sciences; Bone Marrow - chemistry; Cell Adhesion Molecules - genetics; Chromatography - methods; Colorectal Neoplasms - blood; Colorectal Neoplasms - genetics; Colorectal Neoplasms - pathology; Epithelial Cell Adhesion Molecule; Gastroenterology. Liver. Pancreas. Abdomen; Humans; Medical sciences; Neoplasm Metastasis; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - analysis; RNA, Messenger - blood; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Tumors; Human; Rectal disease; Colorectal cancer; Cell adhesion molecule; Malignant tumor; Metastasis; Method; Ep-CAM. circulating tumor cells; Blood; Colonic disease; Cancerology; Messenger RNA; Micrometastasis; Bone marrow; Digestive diseases; Intestinal disease; Advanced stage; micrometastasis. colorectal cancer; Diagnosis; Tumor cell
• ISSN: 0250-7005; 1791-7530

Background: The aim of our study was to detect Ep-CAM mRNA in peripheral blood (PB) and bone marrow (BM) samples of patients
with metastatic colorectal cancer, using a novel chromatographic method (molecular strip) for the detection of PCR amplified
product and to confirm the results by the standard method of agarose gel electrophoresis. Patients and Methods: Thirty patients
participated in the present study providing 27 PB and 26 BM samples. PB samples were also obtained from 20 healthy volunteers.
PCR products were detected using the molecular strip and the results were confirmed by 1.5% agarose gel electrophoresis. Results:
In total 26 out of 27 blood samples (96%) and 19 out of 26 bone marrow samples (73%) were found positive for Ep-CAM expression
using both methods, whereas none of the 20 healthy controls was found positive for the above marker. Conclusion: Ep-CAM mRNA
is highly expressive in peripheral blood of patients with metastatic colorectal cancer. Its prognostic significance should
be evaluated in patients with resectable carcinomas. The new strip detection method is highly specific for the PCR product
under investigation, reliable, easy to handle, fast and safe for the user.