p53 mutations in formaldehyde-induced nasal squamous cell carcinomas in rats

• Published in: Cancer research (Chicago, Ill.) Vol. 52; no. 21; pp. 6113 - 6116
• Main Authors: RECIO, L, SISK, S, PLUTA, L, BERMUDEZ, E, GROSS, E. A, ZHUCHU CHEN, MORGAN, K, WALKER, C
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA American Association for Cancer Research 01-11-1992
• Subjects: Animal tumors. Experimental tumors; Animals; Base Sequence; Biological and medical sciences; Carcinoma, Squamous Cell - chemically induced; Carcinoma, Squamous Cell - genetics; DNA, Neoplasm - analysis; Experimental head and neck tumors. Experimental orbital tumors; Formaldehyde; Genes, p53 - genetics; Medical sciences; Molecular Sequence Data; Mutation - genetics; Nose Neoplasms - chemically induced; Nose Neoplasms - genetics; Polymerase Chain Reaction; Rats; Rats, Inbred F344; Tumors; Squamous cell carcinoma; Rat; Nucleotide sequence; Rodentia; Exploration; Malignant tumor; Formaldehyde; Inhalation; Carcinogen; Vertebrata; Mammalia; Animal; Nose; Point mutation; ENT disease; Mechanism of action
• EISSN: 1538-7445
• ISSN: 0008-5472

Formaldehyde induces squamous cell carcinomas in the nasal passages of rats following chronic inhalation exposure at concentrations of > or = 10 ppm. We have examined the complementary DNA of the tumor suppressor gene p53 from 11 primary formaldehyde-induced tumors for mutation using DNA sequence analysis. A polymerase chain reaction-amplified fragment of the rat p53 complementary DNA containing the evolutionarily conserved regions II-V was directly sequenced from each tumor. Point mutations in the p53 complementary DNA sequence were found in 5 of 11 of the tumors analyzed. These data demonstrate p53 point mutations in formaldehyde-induced squamous cell carcinomas and indicate a common alteration in certain rat and human squamous cell carcinomas of the respiratory tract.