Loss of annexin 1 correlates with early onset of tumorigenesis in esophageal and prostate carcinoma

• Published in: Cancer research (Chicago, Ill.) Vol. 60; no. 22; pp. 6293 - 6297
• Main Authors: PAWELETZ, Cloud P, ORNSTEIN, David K, WANG, Quan-Hong, NAN HU, LINEHAN, W. Marston, TAYLOR, Phillip R, LIOTTA, Lance A, EMMERT-BUCK, Michael R, PETRICOIN, Emanuel F, ROTH, Mark J, BICHSEL, Verena E, GILLESPIE, John W, CALVERT, Valerie S, VOCKE, Cathy D, HEWITT, Stephen M, DURAY, Paul H, HERRING, Judi
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA American Association for Cancer Research 15-11-2000
• Subjects: Adenocarcinoma - metabolism; Annexin A1 - biosynthesis; Annexin A1 - metabolism; Biological and medical sciences; Blotting, Western; Carcinoma, Squamous Cell - metabolism; Dissection - methods; Epithelium - metabolism; Esophageal Neoplasms - metabolism; Esophagus; Esophagus - metabolism; Gastroenterology. Liver. Pancreas. Abdomen; Humans; Immunohistochemistry; Longitudinal Studies; Male; Medical sciences; Nephrology. Urinary tract diseases; Precancerous Conditions - metabolism; Prostate - metabolism; Prostatic Neoplasms - metabolism; Tumors; Tumors of the urinary system; Urinary tract. Prostate gland; Human; Urinary system disease; Carcinoma; Prostate disease; Microdissection; Pathogenesis; Gene product; Tumoral tissue; Esophageal disease; Early stage; Malignant tumor; Epithelium; Gene expression; Carcinogenesis; In vitro; Esophagus; Digestive diseases; Prostate
• ISSN: 0008-5472; 1538-7445

Annexin I protein expression was evaluated in patient-matched longitudinal study sets of laser capture microdissected normal, premalignant, and invasive epithelium from human esophageal squamous cell cancer and prostatic adenocarcinoma. In 25 esophageal cases (20 by Western blot and 5 by immunohistochemistry) and 17 prostate cases (3 by Western blot and 14 by immunohistochemistry), both tumor types showed either complete loss or a dramatic reduction in the level of annexin I protein expression compared with patient-matched normal epithelium (P < or = 0.05). Moreover, by using Western blot analysis of laser capture microdissected, patient-matched longitudinal study sets of both tumor types, the loss of protein expression occurred in premalignant lesions. Concordance of this result with immunohistochemical analysis suggests that annexin I may be an essential component for maintenance of the normal epithelial phenotype. Additional studies investigating the mechanism(s) and functional consequences of annexin I protein loss in tumor cells are warranted.