Indirect method for quantifying the content of photosynthetic pigments in genotypes of dwarf elephant grass/Metodo indireto de quantificacao do conteudo de pigmentos fotossinteticos em genotipos de capim-elefante anao

• Published in: Acta scientiarum. Animal sciences Vol. 35; no. 1; p. 43
• Main Authors: Araujo, Saulo Alberto do Carmo, Vasquez, Hernan Maldonado, Netto, Alena Torres, Campostrini, Eliemar, Deminicis, Bruno Borges, Lima, Erico da Silva
• Format: Journal Article
• Language: Spanish
• Published: Universidade Estadual de Maringa 01-01-2013
• Subjects: Analysis; Carotenoids; Chlorophyll; Genotype; Methods; Photosynthesis; Plant physiology
• ISSN: 1806-2636
• DOI: 10.4025/actascianimsci.v35i1.15178

Equations that indirectly associate the rates obtained by chlorophyll meter SPAD-502 to chlorophyll and carotenoids rates of dwarf elephant grass genotype are provided. Leaves of the genotype of dwarf elephant grass were employed. After being collected, they were transported in light-protected styrofoam boxes to the Plant Physiology Laboratory of UENF. Leaf discs of the known area were then extracted. An average of 5 readings/leaf disc were undertaken by portable chlorophyll meter SPAD-502, with 6 discs for each range and distributed according to the following scale: 0-10; 10-20; 20-30; 30-40; 4050; 50-60. Chlorophyll meter SPAD-502 provides a quick and efficient estimate of total chlorophyll, chlorophyll-a and chlorophyll-b in genotypes of dwarf elephant grass. Keywords: carotenoids, chlorophyll-a, chlorophyll-b, chlorophyllometer, Pennisetum purpureum. O objetivo deste ensaio foi determinar equacoes que indiretamente associem os valores obtidos no medidor de clorofila SPAD-502 aos teores de clorofila e carotenoides de genotipos de capim-elefante anao. Folhas de genotipos de capim-elefante anao foram utilizadas e, depois de coletadas, foram transportadas em caixas de isopor, protegidas da luz, ate o laboratorio de Fisiologia Vegetal da UENF. Posteriormente, os discos foliares de area conhecida foram extraidos. Com o auxilio do medidor portatil de clorofila (MPC) SPAD-502, foi obtida a media de 5 leituras/disco foliar e utilizaram-se 6 discos para cada intervalo, distribuidos de acordo com a seguinte escala de valores do MPC: 0-10; 10-20; 20-30; 30-40; 4050; 50-60. O medidor de clorofila SPAD-502 possibilitou uma rapida e eficaz estimativa do conteudo de clorofila total, clorofila-a e clorofila-b em genotipos de capim-elefante anao. Palavras-chave: carotenoides, clorofila-a, clorofila-b, clorofilometro, Pennisetum purpureum.