Antibacterial and bacteriostatic potential of coelomic fluid and body paste of Pheretima posthuma contra isolados bacterianos clinicos resistentes a ampicilina

• Published in: Brazilian journal of biology Vol. 83
• Main Authors: Hussain, M, Liaqat, I, Ali, N.M, Arshad, N, Hanif, U, Sajjad, S, Sardar, A.A, Awan, U.F, Khan, F.S, Slahuddin
• Format: Journal Article
• Language: English
• Published: Association of the Brazilian Journal of Biology 01-01-2023
• Subjects: Pakistán
• ISSN: 1519-6984
• DOI: 10.1590/1519-6984.247016

Pheretima posthuma (Vaillant, 1868), a native earthworm of Pakistan and Southeast Asia, has wide utilization in vermicomposting and bioremediation process. In this study, P. posthuma coelomic fluid (PCF) and body paste (PBP) was evaluated as antibacterial agent against ampicillin (AMP) resistant five Gram positive and four Gram negative clinical isolates. The antibacterial effect of different doses (i.e. 25-100 [micro]g/ml) of PCF and PBP along with AMP and azithromycin (AZM) (negative and positive controls, respectively) were observed through disc diffusion and microdilution methods. All nine clinical isolates were noticed as AMP resistant and AZM sensitive. Antibacterial effects of PCF and PBP were dose dependent and zone of inhibitions (ZI) against all clinical isolates were between 23.4 [+ or - ] 0.92 to 0 [+ or -] 00 mm. The sensitivity profile of PCF and PBP against clinical isolates was noticed as 44.44 and 55.56%, respectively. Both PCF and PBP showed bacteriostatic (BTS) action against S. aureus, S. pyogenes, K. pneumonia, N. gonorrhoeae. Moreover, the cumulative BTS potential of PCF and PBP against all isolates was 66.67 and 55.56%, respectively. The MICs of PCF and PBP were ranged from 50-200 [micro]g/ml against selected isolates. The bacterial growth curves indicated that PCF and PBP inhibited the growth of all isolates at their specific MIC concentrations. However, PBP has better antibacterial potential compared to PCF against selected isolates. Therefore, it is concluded that both PCF and PBP of P. posthuma possess antibacterial and BTS potential against ampicillin resistant clinical isolates. This organism might be considered as a second choice of antibacterial agents and can further be utilized in pharmaceutical industries for novel drug manufacturing by prospecting bioactive potential agents. Keywords: earthworm paste, minimum inhibitory concentration, bacterial growth curves, resistant bacteria. Pheretima posthuma (Vaillant, 1868), uma minhoca nativa do Paquistao e sudeste da Asia, tem ampla utilizacao em processos de vermicompostagem e biorremediacao. Neste estudo, o fluido celomico de P. posthuma (PCF) e a pasta corporal (PBP) foram avaliados como agente antibacteriano contra cinco isolados clinicos Gram-positivos e quatro Gram-negativos resistentes a ampicilina (AMP). O efeito antibacteriano de diferentes doses (ou seja, 25-100 pg / ml) de PCF e PBP juntamente com AMP e azitromicina (AZM) (controles negativo e positivo, respectivamente) foi observado por meio de metodos de difusao em disco e microdiluicao. Todos os nove isolados clinicos foram notados como resistentes a AMP e sensiveis a AZM. Os efeitos antibacterianos de PCF e PBP foram dependentes da dose e a zona de inibicao (ZI) contra todos os isolados clinicos foi entre 23,4 [+ or -] 0,92 a 0 [+ or -] 00 mm. O perfil de sensibilidade do PCF e PBP contra isolados clinicos foi observado como 44,44% e 55,56%, respectivamente. Tanto o PCF quanto o PBP mostraram acao bacteriostatica (BTS) contra S. aureus, S. pyogenes, K. pneumonia, N. gonorrhoeae. Alem disso, o potencial BTS cumulativo de PCF e PBP contra todos os isolados foi de 66,67% e 55,56%, respectivamente. Os MICs de PCF e PBP variaram de 50-200 [micro]g / ml contra isolados selecionados. As curvas de crescimento bacteriano indicaram que o PCF e o PBP inibiram o crescimento de todos os isolados em suas concentracOes especificas de MIC. No entanto, PBP tem melhor potencial antibacteriano em comparacao com PCF contra isolados selecionados. Portanto, conclui-se que tanto o PCF quanto o PBP de P. posthuma possuem potencial antibacteriano e BTS contra isolados clinicos resistentes a ampicilina. Esse organismo pode ser considerado como uma segunda escolha de agentes antibacterianos e pode ainda ser utilizado nas industrias farmaceuticas para a fabricacao de novos medicamentos por meio da prospeccao de agentes com potencial bioativo. Palavras-chave: pasta de minhoca, concentracao inibitoria minima, curvas de crescimento bacteriano, bacterias resistentes.