In Silico Analysis of the Ga[sup.3+]/Fe[sup.3+] Competition for Binding the Iron-Scavenging Siderophores of IP. aeruginosa/I—Implementation of Three Gallium-Based Complexes in the “Trojan Horse” Antibacterial Strategy

• Published in: Biomolecules (Basel, Switzerland) Vol. 14; no. 4
• Main Authors: Kircheva, Nikoleta, Dobrev, Stefan, Petkova, Vladislava, Yocheva, Lyubima, Angelova, Silvia, Dudev, Todor
• Format: Journal Article
• Language: English
• Published: MDPI AG 01-04-2024
• Subjects: Biotechnological microorganisms; Evaluation; Gallium; Health aspects; Iron; Microbial biotechnology; Physiological aspects; Pseudomonas aeruginosa; Siderophores (Microbiology); Bulgaria
• ISSN: 2218-273X; 2218-273X
• DOI: 10.3390/biom14040487

The emergence of multidrug-resistant (MDR) microorganisms combined with the ever-draining antibiotic pipeline poses a disturbing and immensely growing public health challenge that requires a multidisciplinary approach and the application of novel therapies aimed at unconventional targets and/or applying innovative drug formulations. Hence, bacterial iron acquisition systems and bacterial Fe[sup.2+/3+] -containing enzymes have been identified as a plausible target of great potential. The intriguing “Trojan horse” approach deprives microorganisms from the essential iron. Recently, gallium’s potential in medicine as an iron mimicry species has attracted vast attention. Different Ga[sup.3+] formulations exhibit diverse effects upon entering the cell and thus supposedly have multiple targets. The aim of the current study is to specifically distinguish characteristics of great significance in regard to the initial gallium-based complex, allowing the alien cation to effectively compete with the native ferric ion for binding the siderophores pyochelin and pyoverdine secreted by the bacterium P. aeruginosa. Therefore, three gallium-based formulations were taken into consideration: the first-generation gallium nitrate, Ga(NO[sub.3] )[sub.3] , metabolized to Ga[sup.3+] -hydrated forms, the second-generation gallium maltolate (tris(3-hydroxy-2-methyl-4-pyronato)gallium), and the experimentally proven Ga carrier in the bloodstream—the protein transferrin. We employed a reliable in silico approach based on DFT computations in order to understand the underlying biochemical processes that govern the Ga[sup.3+] /Fe[sup.3+] rivalry for binding the two bacterial siderophores.