Biological effects of sol–gel derived ZrO2 and SiO2/ZrO2 coatings on stainless steel surface—In vitro model using mesenchymal stem cells

• Published in: Journal of biomaterials applications Vol. 29; no. 5; pp. 699 - 714
• Main Authors: Śmieszek, Agnieszka, Donesz-Sikorska, Anna, Grzesiak, Jakub, Krzak, Justyna, Marycz, Krzysztof
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01-11-2014
• Subjects: Adipocytes - cytology; Adipose Tissue - cytology; Austenitic stainless steels; Biocompatibility; Biocompatible Materials - chemistry; Biomaterials; Biomedical materials; Bone marrow; Bone Marrow Cells - cytology; Cell Differentiation; Cell Proliferation; Cell Survival; Coated Materials, Biocompatible - chemistry; Coatings; Gene Expression Regulation; Heat resistant steels; Humans; Materials Testing; Mesenchymal Stromal Cells - cytology; Microscopy, Atomic Force; Microscopy, Electron, Scanning; Osteocalcin - analysis; Osteopontin - analysis; Phase Transition; Silicon dioxide; Silicon Dioxide - chemistry; Spectrometry, X-Ray Emission; Spectrum Analysis, Raman; Stainless Steel - chemistry; Surface Properties; Surgical implants; Wettability; Zirconium - chemistry; Zirconium dioxide; osteoinductive properties; biocompatibility; Sol–gel coatings; surface characterization; mesenchymal stem cells
• ISSN: 0885-3282; 1530-8022
• DOI: 10.1177/0885328214545095

The objective of this study was to determine biocompatibility of zirconia-based coatings obtained by the sol–gel method. Two matrices, ZrO2 and SiO2/ZrO2, were created and applied on stainless steel type 316L with dip-coating technique. The morphology and topography of biomaterials’ surface were characterized using energy-dispersive X-ray spectroscopy and atomic force microscopy, while chemical composition was analyzed by Raman spectroscopy. Additionally, wettability and surface free energy were characterized. Biocompatibility of obtained biomaterials was evaluated using an in vitro model employing mesenchymal stem cells (MSCs) of adipose and bone marrow origin. Biological analysis included determination of proliferation activity and morphology of MSCs in cultures on synthesized biomaterials. Osteoinductive properties of biomaterials were determined both in non-osteogenic, as well as osteogenic conditions. The results showed that investigated biomaterials exerted different impact on MSCs. Biomaterial with ZrO2 layer was more biocompatible for adipose-derived MSCs, while SiO2/ZrO2 layer promoted proliferation of bone marrow derived MSCs. Moreover, hybrid coating exhibited greater osteoinductive properties than ZrO2 coating, both on cultures with adipose-derived stromal (stem) cells and bone marrow stromal cells. Observed biological effects may result not only from different chemical composition, but also from diverse wettability. The ZrO2 coating was characterized as hydrophobic layer, while SiO2/ZrO2 exhibited hydrophilic properties. The results obtained suggest that behavior of MSCs in response to the biomaterial may vary depending on their origin, therefore we postulate, that screening analysis of implants’ biocompatibility, should incorporate model applying both adipose- and bone marrow derived MSCs.