An Interdisciplinary Approach for Designing Kinetic Models of the Ras/MAPK Signaling Pathway

We present in this article a methodology for designing kinetic models of molecular signaling networks, which was exemplarily applied for modeling one of the Ras/MAPK signaling pathways in the mouse Y1 adrenocortical cell line. The methodology is interdisciplinary, that is, it was developed in a way...

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Bibliographic Details
Published in:	Methods in molecular biology (Clifton, N.J.) Vol. 1636; p. 455
Main Authors:	Reis, Marcelo S, Noël, Vincent, Dias, Matheus H, Albuquerque, Layra L, Guimarães, Amanda S, Wu, Lulu, Barrera, Junior, Armelin, Hugo A
Format:	Journal Article
Language:	English
Published:	United States 2017
Subjects:	Algorithms Animals Biomarkers Cell Line Computational Biology - methods Enzyme-Linked Immunosorbent Assay Kinetics Mice Mitogen-Activated Protein Kinases - metabolism Models, Biological Phosphorylation ras Proteins - metabolism Reproducibility of Results Signal Transduction Western blot Model simplification Ras Nonlinear optimization Cell signaling pathways Model-fitting analysis MAPK Ordinary differential equation Differential-algebraic equation ELISA ERK
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Summary:	We present in this article a methodology for designing kinetic models of molecular signaling networks, which was exemplarily applied for modeling one of the Ras/MAPK signaling pathways in the mouse Y1 adrenocortical cell line. The methodology is interdisciplinary, that is, it was developed in a way that both dry and wet lab teams worked together along the whole modeling process.
ISSN:	1940-6029
DOI:	10.1007/978-1-4939-7154-1_28