An Approach to the Identification and Phylogenetic Analysis of Emerging and Hemorrhagic Fever Viruses

An Approach to the Identification and Phylogenetic Analysis of Emerging and Hemorrhagic Fever Viruses

An important aspect of virological surveillance is the identification of the detected viruses. Broad surveillance, that typically employs deep sequencing of collected tissue samples, provides the investigator with many sequence files constructed from overlapping stretches of DNA sequences. Directed...

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Bibliographic Details
Published in:Methods in molecular biology (Clifton, N.J.) Vol. 1604; p. 33
Main Authors: Díaz, Francisco J, Paternina, Luis E, Rodas, Juan David
Format: Journal Article
Language:English
Published: United States 2018
Subjects:
Computational Biology
DNA, Complementary - genetics
Hantaan virus - genetics
Humans
Phylogeny
Phylogenetics
Alignment
Databases
Genetic trees
Bioinformatics
Hemorrhagic viruses
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Summary:An important aspect of virological surveillance is the identification of the detected viruses. Broad surveillance, that typically employs deep sequencing of collected tissue samples, provides the investigator with many sequence files constructed from overlapping stretches of DNA sequences. Directed surveillance for viruses of a specific taxonomic group provides the investigator with sequence files from cDNA amplified using specific primers to conserved viral regions. Here we will describe general approaches to identify hemorrhagic viral agents through phylogenetic analysis of cDNA sequences obtained during surveillance activities.
ISSN:1940-6029
DOI:10.1007/978-1-4939-6981-4_2