Antigen of 49.6-kDa subunitpili protein of Helicobacter pylori as a potential biomarker for early and rapid detection of the infection

• Published in: Veterinary World Vol. 12; no. 6; pp. 769 - 773
• Main Authors: Suharsono, Hamong, Muttaqin, Zainul, Tenaya, I Wayan Masa, Agustina, Kadek Karang, Prawiro, Sumarno Retro
• Format: Journal Article
• Language: English
• Published: India Veterinary World 01-06-2019
• Subjects: Analysis; Antibodies; Antigens; Cancer; Carcinoma; Cytochemistry; Gastritis; Health aspects; Helicobacter infections; Helicobacter pylori; Immune response; Immunoglobulin G; Immunoglobulins; Infection; Lymphomas; Peptic ulcer; Proteins; Stomach cancer; Tumors; Ulcers; Indonesia; 49.6-kDa pili protein; Helicobacter pylori; immunochromatographic test
• ISSN: 0972-8988; 2231-0916
• DOI: 10.14202/vetworld.2019.769-773

infection has been identified as a major cause of peptic ulcer diseases, including gastric and duodenal ulcers, gastritis, chronic and gastric carcinoma, and even gastric lymphoma.
studies using Western blotting analysis, hemagglutination test, adherence inhibition assays, and immunocytochemical staining revealed that the 49.6-kDa subunit pili protein of
was considered an immunogenic protein. This study aimed to develop a serological diagnostic test using 49.6 kDa for detecting antibodies against
proteins in an early phase of the infection.
An in-house immunochromatographic test (ICT) kit was developed and used to test a panel of sera sample obtained from a randomly selected symptomatic patient, in which 40 sera were
positive and 40 sera were
negative.
The results showed that ICT with 49.6 kDa as an antigen was highly sensitive and specific for detecting anti-
immunoglobulin G antibodies in human serum, with a high negative predictive value.
The developed test could be used to exclude
infection in symptomatic patients.