Purification of GNA-Related Lectins from Natural Sources

The Galanthus nivalis lectin, abbreviated as GNA, is the model protein for a large group of mannose-binding lectins. Here, we describe the purification of GNA starting from dry bulbs. Using a combination of ion exchange chromatography and affinity chromatography on mannose-Sepharose, a highly pure p...

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Bibliographic Details
Published in:	Methods in molecular biology (Clifton, N.J.) Vol. 2132; p. 413
Main Author:	Van Damme, Els J M
Format:	Journal Article
Language:	English
Published:	United States 2020
Subjects:	Chromatography, Affinity Chromatography, Ion Exchange Galanthus - metabolism Mannose - chemistry Mannose-Binding Lectins - isolation & purification Plant Lectins - isolation & purification Plant Roots - metabolism Sepharose - chemistry GNA Agglutination activity Glycan binding Plant lectin Snowdrop
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Summary:	The Galanthus nivalis lectin, abbreviated as GNA, is the model protein for a large group of mannose-binding lectins. Here, we describe the purification of GNA starting from dry bulbs. Using a combination of ion exchange chromatography and affinity chromatography on mannose-Sepharose, a highly pure preparation of GNA can be obtained.
ISSN:	1940-6029
DOI:	10.1007/978-1-0716-0430-4_40