1,3- beta -glucanases from Streptomyces sp.1228 lytic enzyme system

Four 1,3- beta -glucanases GI, GII, GIV and GVIII from a culture filtrate of Streptomyces sp.1228 were purified by anion exchange chromatography using DEAE-Sepharose Cl-6B or DEAE-Cellulose, gel filtration on Bio-Gel P-200 or Sephacryl S- 200, Amicon ultrafiltration and preparative PAGE. The M sub(r...

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Bibliographic Details
Published in:	Biotechnology letters Vol. 11; no. 4; pp. 281 - 286
Main Authors:	Bielecki, S, Wnuk, M, Szczesna, M, Bobowicz-Lassocinska, T, Antczak, T, Galas, E
Format:	Journal Article
Language:	English
Published:	01-01-1989
Subjects:	1,3- beta -glucanase
Online Access:	Get full text
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Summary:	Four 1,3- beta -glucanases GI, GII, GIV and GVIII from a culture filtrate of Streptomyces sp.1228 were purified by anion exchange chromatography using DEAE-Sepharose Cl-6B or DEAE-Cellulose, gel filtration on Bio-Gel P-200 or Sephacryl S- 200, Amicon ultrafiltration and preparative PAGE. The M sub(r) of these enzymes were 19,000, 74,000, 78,000 and 56,000 respectively. The glucanase GVIII consisted of two subunits. The optimal catalytic activity of the purified preparations was at 50-55 degree C and pH 5.5 - 6.0. The enzymes were also most stable at this pH. Both glucanases GI and GVIII were characterized by high thermostability.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0141-5492
DOI:	10.1007/BF01031578