Experimental Lactococcus garvieae infection in rainbow trout, Oncorhynchus mykiss, Walbaum 1792: a comparative histopathological and immunohistochemical study

• Published in: Journal of fish diseases Vol. 37; no. 5; pp. 481 - 495
• Main Authors: Avci, H, Birincioglu, S S, Tanrikul, T T, Epikmen, E T, Metin, N, Avsever, M L
• Format: Journal Article
• Language: English
• Published: England Blackwell Science 01-05-2014; Blackwell Publishing Ltd
• Subjects: adults; Animals; bacteria; bacterial antigens; Body Size; DNA, Bacterial - genetics; eyes; fish; Fish Diseases - microbiology; Fish Diseases - pathology; fish farms; fluorescent antibody technique; Gram-Positive Bacterial Infections - microbiology; Gram-Positive Bacterial Infections - pathology; Gram-Positive Bacterial Infections - veterinary; heart; histopathology; Immersion; immunohistochemistry; Injections, Intraperitoneal - veterinary; kidneys; Lactococcus - physiology; Lactococcus garvieae; liver; Molecular Sequence Data; mortality; Oncorhynchus mykiss; Oncorhynchus mykiss - physiology; pathology; peritoneum; polymerase chain reaction; Polymerase Chain Reaction - veterinary; rainbow trout; ribosomal DNA; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA - veterinary; spleen; swim bladder; pathology; immunohistochemistry; Lactococcus garvieae; rainbow trout
• ISSN: 0140-7775; 1365-2761
• DOI: 10.1111/jfd.12132

The aim of this study was to induce Lactococcus garvieae infection in young and adult fish through different routes [intraperitoneal (IP) and immersion (IM)] and to investigate the pathogenesis and histopathological and immunohistochemical findings comparatively. For this purpose, a total of 180 rainbow trout (90 young, 20 ± 5 g and 90 adult, 80 ± 10 g) obtained from a commercial fish farm were used. The fish were divided into eight groups, four experimental groups (Young‐Adult IP groups and Young‐Adult IM groups, each contain 30 fish) and four control groups (Young‐Adult IP Control groups and Young‐Adult IM control groups, each contain 15 fishes). The experimental study was conducted using L. garvieae, and confirmatory identification was performed by PCR. The sequence result of the PCR amplicon of 16S rDNA from isolate L. garvieae LAC1 was determined and deposited in the GenBank database under accession number KC883976. Fish in the IP groups were intraperitoneally administered an inoculate containing 10⁶ cfu mL⁻¹ bacteria 0.1 mL. In the IM groups, fish were kept in inoculated water containing 10⁸ cfu mL⁻¹ bacteria for 20 min. Mortality as well as clinical and pathological findings was recorded daily, and significant differences in macroscopic and microscopic results were observed between the IP and IM administration groups. All tissue samples were immunohistochemically stained by the avidin‐biotin‐peroxidase complex and immunofluorescence (IF) methods using polyclonal antibody to detect L. garvieae antigens. In immunoperoxidase staining in the IP groups, positive reactions to bacterial antigens were most commonly seen in the spleen, kidney, heart, liver, peritoneum and swim bladder. In the IM groups, bacterial antigens were most commonly found in the eye, gill, spleen and kidney. In the IF method, the distribution of antigens in tissue and organs was similar to the reactions with immunoperoxidase staining. Finally, in this experimental study, an important correlation was seen between the distribution of L. garvieae antigens and lesions developing in many organ and tissues.