Development and validation of a method for the quantitative determination of aflatoxin contaminants in Maytenus ilicifolia by HPLC with fluorescence detection

• Published in: Phytochemical analysis Vol. 16; no. 4; pp. 267 - 271
• Main Authors: Braga, S.M.L.F.M, Medeiros, F. D. de, Oliveira, E. de J, Macedo, R.O
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01-07-2005
• Subjects: aflatoxin B1; aflatoxin B2; aflatoxin G1; aflatoxin G2; aflatoxins; Aflatoxins - analysis; Brazil; Chromatography, Affinity; Chromatography, High Pressure Liquid - methods; contaminants; herbaceous plants; high performance liquid chromatography; HPLC; immunoaffinity column; Maytenus; Maytenus - chemistry; Maytenus ilicifolia; medicinal plants; method validation; Plant Extracts - chemistry; quantitative analysis; Reproducibility of Results; Spectrometry, Fluorescence; Brazil
• ISSN: 0958-0344; 1099-1565
• DOI: 10.1002/pca.837

A method for the quantification of aflatoxins B1, G1, B2 and G2 in the medicinal herb Maytenus ilicifolia was developed and validated. The method used immunoaffinity columns for sample clean-up and HPLC with fluorescence detection without any derivatisation step. The method showed good inter-day accuracy (bias values in the range 4.5-10.7%) and precision (5-16% RSD) when applied to the determination of levels of aflatoxins ranging from 7 to 20 ppb in the plant material. The detection limits for samples of the plant material spiked with aflatoxins were 3.5 ng/g for B1 and G1 and 0.1 ng/g for B2 and G2. The method was successfully applied to commercial samples of Maytenus ilicifolia for the screening of aflatoxin contaminants.