Genome-wide analysis of Kluyveromyces lactis in wild-type and rag2 mutant strains

• Published in: Genome Vol. 47; no. 5; p. 970
• Main Authors: Becerra, Manuel, Tarrío, Nuria, González-Siso, M Isabel, Cerdán, M Esperanza
• Format: Journal Article
• Language: English
• Published: Canada 01-10-2004
• Subjects: Fructose - metabolism; Gene Expression Profiling; Gene Expression Regulation, Fungal - genetics; Gene Expression Regulation, Fungal - physiology; Genome, Fungal; Glucose - metabolism; Glucose-6-Phosphate Isomerase - genetics; Glucose-6-Phosphate Isomerase - metabolism; Kluyveromyces - enzymology; Kluyveromyces - genetics; Mutation - genetics; NADP - metabolism; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics
• ISSN: 0831-2796
• DOI: 10.1139/g04-039

The use of heterologous DNA arrays from Saccharomyces cerevisiae has been tested and revealed as a suitable tool to compare the transcriptomes of S. cerevisiae and Kluyveromyces lactis, two yeasts with notable differences in their respirofermentative metabolism. The arrays have also been applied to study the changes in the K. lactis transcriptome owing to mutation in the RAG2 gene coding for the glycolytic enzyme phosphoglucose isomerase. Comparison of the rag2 mutant growing in 2% glucose versus 2% fructose has been used as a model to elucidate the importance of transcriptional regulation of metabolic routes, which may be used to reoxidize the NADPH produced in the pentose phosphate pathway. At this transcriptional level, routes related to the oxidative stress response become an interesting alternative for NADPH use.