Nuclear genome constitution and other characteristics of somatic hybrids between dihaploid Solanum acaule and tetraploid S. tuberosum

• Published in: Euphytica Vol. 102; no. 2; pp. 239 - 246
• Main Authors: Yamada, T. (Kobe Univ., Nada, Kobe (Japan). Graduate School of Science and Technology, Div. of Science of Biological Resources), Hosaka, K, Nakagawa, K, Kaide, N, Misoo, S, Kamijima, O
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01-01-1998; Springer Nature B.V
• Subjects: Agronomy. Soil science and plant productions; ANTHER CULTURE; Biological and medical sciences; Chromosomes; CULTIVO DE ANTERAS; CULTURE D'ANTHERE; DIHAPLOIDIA; DIHAPLOIDIE; DIHAPLOIDY; Fundamental and applied biological sciences. Psychology; GENETIC MARKERS; Genetics and breeding of economic plants; HIBRIDACION INTERESPECIFICA; HIBRIDOS SOMATICOS; HYBRIDATION INTERSPECIFIQUE; HYBRIDE SOMATIQUE; Hybrids; Interspecific and intergeneric hybridization, introgressions; INTERSPECIFIC HYBRIDIZATION; MARCADORES GENETICOS; MARQUEUR GENETIQUE; Plant breeding: fundamental aspects and methodology; Pollen; Probes; RFLP; SOLANUM TUBEROSUM; SOMATIC HYBRIDS; TETRAPLOIDIA; TETRAPLOIDIE; TETRAPLOIDY; Chromosome pairing; Solanum tuberosum; Cell nucleus; Dihaploidy; Crossability; Characterization; Tetraploidy; Restriction fragment length polymorphism; Fertility; Dicotyledones; Angiospermae; Interspecific hybridization; Somatic hybrid; Spermatophyta; Pollen; Tuber plant; Solanaceae; Genome
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1023/A:1018325529119

Eleven somatic hybrids (2n = 68 to 74) obtained between S. tuberosum ssp. tuberosum cv. Dejima (2n = 48) and ATDH-1 (2n = 24), an anther-culture-derived dihaploid of S. acaule (Yamada et al., 1997), were characterized by nuclear RFLP markers using 49 single-copy DNA probes distributed throughout the potato genome (2 to 6 probes per chromosome). One of the somatic hybrids, DA8-2, had 72 chromosomes and all the Dejima- and ATDH-1-specific markers (124 and 103 bands, respectively), suggesting the presence of a whole set of both parental chromosomes. The other somatic hybrids lost varying numbers of markers up to seventeen. The pattern of the loss of markers indicated the elimination of five chromosomes among four somatic hybrids. A nucleolar organizer region of chromosome 2 was often eliminated in the somatic hybrids. The somatic hybrids studied here had higher frequencies of multivalent formation than the S. tuberosum parent. They had reasonably good seed set when pollinated with S. tuberosum pollen. Hence, homoeologous recombination between S. acaule and S. tuberosum chromosomes is possible and useful traits from S. acaule may be transferred to the S. tuberosum gene pool.[PUBLICATION ABSTRACT]