Simple and rapid method for the selective detection of individual lipoxygenase isozymes in soybean seeds

• Published in: Journal of agricultural and food chemistry Vol. 43; no. 3; pp. 742 - 747
• Main Authors: Suda, I. (Kyushu National Agricultural Experiment Station, Kumamoto, Japan.), Hajika, M, Nishiba, Y, Furata, S, Igita, K
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01-03-1995
• Subjects: Agronomy. Soil science and plant productions; ANALYTICAL METHODS; BETA-CAROTENE; Biological and medical sciences; BLANCHISSEMENT; BLANQUEADURA; BLEACHING; CAROTENOIDE; CAROTENOIDES; CAROTENOIDS; DESINFECTANT; DESINFECTANTES; DISINFECTANTS; ENSAYO; Fundamental and applied biological sciences. Psychology; Genetics and breeding of economic plants; GLYCINE MAX; ISOENZIMAS; ISOENZYME; ISOENZYMES; LIPOXIGENASA; LIPOXYGENASE; METHYLENE BLUE; MUTANT; MUTANTES; MUTANTS; SCREENING; SOJA; SOYBEANS; TECHNIQUE ANALYTIQUE; TECNICAS ANALITICAS; TESTAGE; TESTING; Varietal selection. Specialized plant breeding, plant breeding aims; Yield, quality, earliness, varia; Discoloration; Enzyme; Isozyme; Soybean; Varietal selection; Glycine max; Methylene blue; Grain legume; Rapid technique; Leguminosae; Dicotyledones; Angiospermae; Spermatophyta; Oxidoreductases; Detection; Lipoxygenase; Spectrophotometry; Cultivar
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf00051a034

A simple and rapid spectrophotometric and visual judging method was developed for the selective detection of individual lipoxygenase isozymes (L-1, L-2, and L-3) in soybean [Glycine max (L.) Merrill] seeds, based on the bleaching activities of these isozymes in contact with methylene blue and beta-carotene. The method consists of three procedures designated tests I-III. The tests are performed by mixing soybean extract samples or wetted soybean flour with each dye-substrate of test I for the L-1 isozyme, test II for the L-2 isozyme, and test III for the L-3 isozyme, whereupon the presence of individual isozymes contained in the soybean is determined spectrophotometically or visually within 10 or 5 min, respectively. In the visual judging method available as a routine screening test, the total amount of soybean flour required for the three tests is only about 10 mg