Fluorometric riboflavin titration in plasma by riboflavin-binding apoprotein as a method for vitamin B2 status assessment

• Published in: Annals of nutrition and metabolism Vol. 39; no. 6; p. 355
• Main Authors: Kodentsova, V M, Vrzhesinskaya, O A, Spirichev, V B
• Format: Journal Article
• Language: English
• Published: Switzerland 1995
• Subjects: Adolescent; Adult; Apoproteins - blood; Child; Child, Preschool; Female; Flavin-Adenine Dinucleotide - pharmacology; Humans; Male; Middle Aged; Nutritional Status; Riboflavin - blood; Riboflavin - urine; Spectrometry, Fluorescence
• ISSN: 0250-6807
• DOI: 10.1159/000177885

A method for plasma riboflavin determination by riboflavin-binding apoprotein titration has been proposed for vitamin B2 status evaluation. The method is based on the formation of riboflavin-apoprotein complex accompanied by full loss of fluorescence peculiar to free riboflavin. The data obtained have demonstrated a correlation with indicators of the vitamin B2 status such as urinary excretion, erythrocyte content, and stimulation of the glutathione reductase activity by flavin adenine dinucleotide. Levels > 6 ng/ml blood plasma may be considered to be a criterion for normal vitamin B2 supply.