Infection of cultured thin cell layer roots of Lycopersicon esculentum by Meloidogyne incognita

• Published in: Journal of nematology Vol. 23; no. 4; pp. 441 - 445
• Main Authors: Radin, D.N. (Virginia Polytechnic Institute and State University, Blacksburg, VA), Eisenback, J.D
• Format: Journal Article
• Language: English
• Published: United States Society of Nematologists 01-10-1991
• Subjects: CULTIVO DE TEJIDOS; CULTURE DE TISSU; GENOTIPOS; GENOTYPE; GENOTYPES; LYCOPERSICON ESCULENTUM; MELOIDOGYNE INCOGNITA; RACINE; RAICES; RESISTANCE; ROOTS; TISSUE CULTURE; nematode; Meloidogyne incognita; host susceptibility; Lycopersicon esculentum; host resistance; aseptic culture; southern root-knot nematode; tomato
• ISSN: 0022-300X; 2640-396X

A new aseptic culture system for studying interactions between tomato (Lycopersicon esculentum) and Meloidogyne incognita is described. Epidermal thin cell layer explants from peduncles of tomato produced up to 20 adventitious roots per culture in 4-9 days on Murashige &Scoog medium plus kinetin and indole acetic acid. Rooted cultures were transferred to Gamborg's B-5 medium and inoculated with infective second-stage juveniles. Gall formation was apparent 5 days after inoculation and egg production by mature females occurred within 25 days at 25 C in the susceptible genotypes Rutgers and Red Alert. Resistant genotypes LA655, LA656, and LA1022 exhibited a characteristic hypersensitive response. This system provides large numbers of cultured root tips for studies on the molecular basis of the host-parasite relationship.